| Insect juvenile hormone resistance gene homology with the bHLH-PAS family of transcriptional regulators. | |
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MedLine Citation:
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PMID: 9501163 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Juvenile hormone analog (JHA) insecticides are relatively nontoxic to vertebrates and offer effective control of certain insect pests. Recent reports of resistance in whiteflies and mosquitoes demonstrate the need to identify and understand genes for resistance to this class of insect growth regulators. Mutants of the Methoprene-tolerant (Met) gene in Drosophila melanogaster show resistance to both JHAs and JH, and previous biochemical studies have demonstrated a mechanism of resistance involving an intracellular JH binding-protein that has reduced ligand affinity in Met flies. We cloned the Met+ gene by transposable P-element tagging and found reduced transcript level in several mutant alleles, showing that underproduction of the normal gene product can lead to insecticide resistance. Transformation of Met flies with a Met+ cDNA resulted in susceptibility to methoprene, indicating that the cDNA encodes a functional Met+ protein. MET shows homology to the basic helix-loop-helix (bHLH)-PAS family of transcriptional regulators, implicating MET in the action of JH at the gene level in insects. This family also includes the vertebrate dioxin receptor, a transcriptional regulator known to bind a variety of environmental toxicants. Because JHAs include a diverse array of chemicals with JH activity, a mechanism whereby they can exert effects in insects through a common pathway is suggested. |
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Authors:
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M Ashok; C Turner; T G Wilson |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 95 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1998 Mar |
Date Detail:
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Created Date: 1998-04-10 Completed Date: 1998-04-10 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2761-6 Citation Subset: IM |
Affiliation:
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Department of Biology, Colorado State University, Fort Collins, CO 80523, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF034859 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Basic Helix-Loop-Helix Transcription Factors Cloning, Molecular DNA Transposable Elements Drosophila Proteins* Drosophila melanogaster / drug effects, genetics* Genes, Insect* Helix-Loop-Helix Motifs* Insecticide Resistance / genetics Insecticides / pharmacology* Juvenile Hormones / agonists Methoprene / pharmacology* Molecular Sequence Data RNA, Messenger / genetics Sequence Homology, Amino Acid Trans-Activators / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Basic Helix-Loop-Helix Transcription Factors; 0/DNA Transposable Elements; 0/Drosophila Proteins; 0/Insecticides; 0/Juvenile Hormones; 0/RNA, Messenger; 0/Resistance to Juvenile Hormone protein, Drosophila; 0/Trans-Activators; 40596-69-8/Methoprene |
| Comments/Corrections | |
Comment In:
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Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):2725-6
[PMID:
9501155
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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