Document Detail


Insect chitinase and chitinase-like proteins.
MedLine Citation:
PMID:  19816755     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Insect chitinases belong to family 18 glycosylhydrolases that hydrolyze chitin by an endo-type of cleavage while retaining the anomeric beta-(1-->4) configuration of products. There are multiple genes encoding chitinases and chitinase-like proteins in all insect species studied using bioinformatics searches. These chitinases differ in size, domain organization, physical, chemical and enzymatic properties, and in patterns of their expression during development. There are also differences in tissue specificity of expression. Based on a phylogenetic analysis, insect chitinases and chitinase-like proteins have been classified into several different groups. Results of RNA interference experiments demonstrate that at least some of these chitinases belonging to different groups serve non-redundant functions and are essential for insect survival, molting or development. Chitinases have been utilized for biological control of insect pests on transgenic plants either alone or in combination with other insecticidal proteins. Specific chitinases may prove to be useful as biocontrol agents and/or as vaccines.
Authors:
Yasuyuki Arakane; Subbaratnam Muthukrishnan
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Review     Date:  2009-10-09
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  67     ISSN:  1420-9071     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  201-16     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Kansas State University, 141 Chalmers Hall, Manhattan, KS 66506-3702, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Chitinase* / chemistry,  classification,  genetics
Conserved Sequence
Gene Expression Regulation, Enzymologic
Insect Control*
Insects / enzymology*
Molecular Sequence Data
Pesticides / metabolism
Plants, Genetically Modified / enzymology,  genetics
Protein Structure, Tertiary
Substrate Specificity
Chemical
Reg. No./Substance:
0/Pesticides; EC 3.2.1.14/Chitinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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