Document Detail


Inositol phosphates in rat atria and the importance of the extraction procedure.
MedLine Citation:
PMID:  8386256     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nature of the inositol phosphates present in adult rat left atria have been examined. Trichloroacetic acid extracts of [3H] inositol-labelled left atria contained the 1 (or 3)-, 2- and 4-isomers of inositol monophosphate, inositol-1,4-bisphosphate, and inositol-1,4,5-trisphosphate. Addition of noradrenaline increased all of these isomers. However, even in the presence of noradrenaline, there was no evidence for the presence of inositol-1,3,4,5-tetrakisphosphate or its dephosphorylation products including inositol-1,3,4-trisphosphate and the 1,3- and 3,4-isomers of inositol bisphosphate. Total accumulation of inositol phosphates in the presence of LiCl was 98% accounted for by dephosphorylation of inositol-1,4,5-trisphosphate and its cyclic isomer. These data indicate that, in intact left atria, metabolism of inositol-1,4,5-trisphosphate is by dephosphorylation and that significant activity of the phosphorylation pathway is not present. When extractions were performed using either acidified chloroform-methanol or perchloric acid, other [3H] inositol-labelled compounds were detected in atrial extracts. One of these chromatographed with ATP and might be mistaken for inositol-1,3,4-trisphosphate if co-chromatography with ATP was used to identify that compound. Another compound had a similar, but not identical chromatographic profile to inositol-1,3,4,5-tetrakisphosphate and is thus likely to be mistaken for that compound. In addition, perchloric acid extraction caused phosphate group migration generating extra isomers of the inositol phosphates and other unidentified [3H]-labelled compounds. Such extraction artifacts are likely especially problematic in studies of intact heart tissue because of the time required to effectively homogenize the tissue. These findings demonstrate the need for caution in interpreting data relating to estimations of inositol phosphates in intact heart tissue.
Authors:
E A Woodcock; K E Anderson
Related Documents :
21630586 - Repellent and insecticidal activities of melia azedarach l. against cotton leafworm, sp...
4005536 - Octanoic acid-induced coma and reticular formation energy metabolism.
1349716 - Esterification of fatty acids by bovine intramuscular and subcutaneous adipose tissues.
17602736 - Structural and rheological characterization of hyaluronic acid-based scaffolds for adip...
23882836 - Biogenic silver nanoparticles with chlorogenic acid as a bioreducing agent.
2276876 - High-performance liquid chromatography of 2,4-disubstituted-5(4h)-oxazolones, anhydride...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  25     ISSN:  0022-2828     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-05-17     Completed Date:  1993-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  215-27     Citation Subset:  IM    
Affiliation:
Baker Medical Research Institute, Prahran, Victoria, Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Artifacts
Chloroform
Heart Atria / chemistry*,  drug effects
Inositol Phosphates / isolation & purification*
Methanol
Norepinephrine / pharmacology
Perchloric Acid
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Trichloroacetic Acid
Chemical
Reg. No./Substance:
0/Inositol Phosphates; 51-41-2/Norepinephrine; 67-56-1/Methanol; 67-66-3/Chloroform; 76-03-9/Trichloroacetic Acid; 7601-90-3/Perchloric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Formation of fetal rat cardiac cell clones by retroviral transformation: retention of select myocyte...
Next Document:  High-fiber diet in the treatment of obesity and hypercholesterolemia.