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INOSITOL ADMINISTRATION REDUCES OXIDATIVE STRESS IN ERYTHROCYTES OF PATIENTS WITH POLYCYSTIC OVARY SYNDROME.
MedLine Citation:
PMID:  22223702     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Objective: Possibly due to a deficiency of insulin mediators, polycystic ovary syndrome (PCOS) is often associated with insulin resistance and hyperinsulinemia, likely responsible for an elevated production of reactive oxygen species (ROS). We investigated oxidative-related alterations in erythrocytes and anti-inflammatory effects of inositol in women with PCOS before and after treatment with myo-inositol (MYO).Methods: 26 normal weight PCOS patients were investigated before and after MYO administration (1200 mg/day for 12 weeks) (n=18) or placebo (n=8) by evaluating serum testosterone, serum androstenedione, fasting serum insulin, fasting serum glucose, insulin area under the curve (AUC) and glucose AUC after oral glucose tolerance test (OGTT) and Homeostasis Model of Assessment - Insulin Resistance (HOMA-IR). In erythrocytes, band 3 tyrosine-phosphorylation (Tyr-P) level, glutathione (GSH) content and glutathionylated proteins (GSSP) were also assessed.Results: Data show that PCOS patients' erythrocytes underwent oxidative stress as indicated by band 3 Tyr-P values, reduced cytosolic glutathione content and increased membrane protein glutathionylation.MYO treatment significantly improved metabolic and biochemical parameters. Significant reductions were found in insulin resistance, serum values of androstenedione and testosterone. A significant association between band 3 Tyr-P levels and insulin AUC was found at baseline, but disappeared after MYO treatment, while a correlation between band 3 Tyr-P and testosterone levels was detected both before and after MYO treatment.Conclusions: PCOS patients suffer from a systemic inflammatory status that induces erythrocyte membrane alterations. Treatment with MYO is effective in reducing hormonal, metabolic and oxidative abnormalities in PCOS patients by improving insulin resistance.
Authors:
Gabriella Donà; Chiara Sabbadin; Cristina Fiore; Marcantonio Bragadin; Francesco Libero Giorgino; Eugenio Ragazzi; Giulio Clari; Luciana Bordin; Decio Armanini
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-5
Journal Detail:
Title:  European journal of endocrinology / European Federation of Endocrine Societies     Volume:  -     ISSN:  1479-683X     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423848     Medline TA:  Eur J Endocrinol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
G Donà, Department of Biological Chemistry, University of Padova, Padova, Italy.
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