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Inositol 1,4,5-trisphosphate-induced calcium release and guanine nucleotide-binding protein-mediated periodic calcium rises in golden hamster eggs.
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MedLine Citation:
PMID:  3123497     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Periodic increases in intracellular free calcium occur upon fertilization of golden hamster eggs (Miyazaki et al. 1986. Dev. Biol. 118:259-267). To investigate the underlying mechanism, inositol 1,4,5-trisphosphate (IP3) and guanine nucleotides were microinjected into the egg while Ca2+ transients were monitored by aequorin luminescence and/or hyperpolarization in the membrane potential, which indicates the exact timing and spatial distribution of the Ca2+ rise. Injection of IP3 induced an immediate Ca2+ transient of 13-18 s in the entire egg. The critical concentration of IP3 was 80 nM in the injection pipette (2 nM in the egg, assuming uniform distribution); the effect was all-or-none. The Ca2+ rise occurred even in Ca-free external medium. Injection of 5 mM GTP or 0.33 mM guanosine-5'-O-(3-thiotriphosphate) (GTP gamma S) (calculated intracellular concentration, 200 or 12 microM, respectively) caused a similar Ca2+ transient with a delay of 160-200 s. More than 50 microM GTP gamma S produced recurring and attenuating Ca2+ transients in a local area of the cytoplasm, with an initial delay of 25-40 s and intervals of 45-60 s. In Ca-free medium the first one to two Ca2+ transients occurred but succeeding ones were absent. Preinjection of guanosine-5'-O-(2-thiodiphosphate) inhibited the occurrence of both GTP gamma S-induced and sperm-induced Ca2+ transients in a dose-dependent manner. Neither pertussis nor cholera toxins had effect. It was proposed that sperm-egg interaction activates a GTP-binding protein that stimulates production of IP3, causing the first one to two Ca releases from internal stores, and also stimulates a pathway for elevation of Ca2+ permeability in the plasma membrane, thereby sustaining the repeated Ca2+ releases.
Authors:
S Miyazaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of cell biology     Volume:  106     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-03-16     Completed Date:  1988-03-16     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  345-53     Citation Subset:  IM    
Affiliation:
Department of Physiology, Jichi Medical School, Tochigi-ken, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aequorin / diagnostic use
Animals
Calcium / physiology*
Cholera Toxin / pharmacology
Cricetinae
Fertilization
GTP-Binding Proteins / physiology*
Guanosine 5'-O-(3-Thiotriphosphate)
Guanosine Diphosphate / analogs & derivatives,  pharmacology
Guanosine Triphosphate / analogs & derivatives,  pharmacology
Inositol 1,4,5-Trisphosphate
Inositol Phosphates / pharmacology,  physiology*
Membrane Potentials
Microinjections
Ovum / physiology*
Periodicity
Sugar Phosphates / physiology*
Thionucleotides / pharmacology
Virulence Factors, Bordetella / pharmacology
Chemical
Reg. No./Substance:
0/Inositol Phosphates; 0/Sugar Phosphates; 0/Thionucleotides; 0/Virulence Factors, Bordetella; 146-91-8/Guanosine Diphosphate; 37589-80-3/Guanosine 5'-O-(3-Thiotriphosphate); 50934-79-7/Aequorin; 71376-97-1/guanosine 5'-O-(2-thiodiphosphate); 7440-70-2/Calcium; 85166-31-0/Inositol 1,4,5-Trisphosphate; 86-01-1/Guanosine Triphosphate; 9012-63-9/Cholera Toxin; EC 3.6.1.-/GTP-Binding Proteins
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 2 Year: 1988
Volume: 106 Issue: 2
First Page: 345 Last Page: 353
ID: 2114965
Publisher Id: 88115584
PubMed Id: 3123497

Inositol 1,4,5-trisphosphate-induced calcium release and guanine nucleotide-binding protein-mediated periodic calcium rises in golden hamster eggs


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