| Inositol 1,3,4,5-tetrakisphosphate and not phosphatidylinositol 3,4-bisphosphate is the probable precursor of inositol 1,3,4-trisphosphate in agonist-stimulated parotid gland. | |
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MedLine Citation:
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PMID: 2432882 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When [3H]inositol-prelabelled rat parotid-gland slices were stimulated with carbachol, noradrenaline or Substance P, the major inositol trisphosphate produced with prolonged exposure to agonists was, in each case, inositol 1,3,4-trisphosphate. Much lower amounts of radioactivity were present in the inositol 1,4,5-trisphosphate fraction separated by anion-exchange h.p.l.c. Analysis of the inositol trisphosphate head group of phosphatidylinositol bisphosphate in [32P]Pi-labelled parotid glands showed the presence of phosphatidylinositol 4,5-bisphosphate, but no detectable phosphatidylinositol 3,4-bisphosphate. Carbachol-stimulated [3H]inositol-labelled parotid glands contained an inositol polyphosphate with the chromatographic properties and electrophoretic mobility of an inositol tetrakisphosphate, the probable structure of which was determined to be inositol 1,3,4,5-tetrakisphosphate. Since an enzyme in erythrocyte membranes is capable of degrading this tetrakisphosphate to inositol 1,3,4-trisphosphate, it is suggested to be the precursor of inositol 1,3,4-trisphosphate in parotid glands. |
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Authors:
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C P Downes; P T Hawkins; R F Irvine |
Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: The Biochemical journal Volume: 238 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1986 Sep |
Date Detail:
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Created Date: 1987-01-29 Completed Date: 1987-01-29 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 501-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbachol / pharmacology Chromatography, High Pressure Liquid Chromatography, Ion Exchange Inositol 1,4,5-Trisphosphate Inositol Phosphates / metabolism* Norepinephrine / pharmacology Parotid Gland / drug effects, metabolism* Rats Substance P / pharmacology Sugar Phosphates / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Inositol Phosphates; 0/Sugar Phosphates; 102850-29-3/inositol-1,3,4,5-tetrakisphosphate; 33507-63-0/Substance P; 51-41-2/Norepinephrine; 51-83-2/Carbachol; 85166-31-0/Inositol 1,4,5-Trisphosphate |
| Comments/Corrections | |
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