Document Detail


Inositol 1,3,4,5-tetrakisphosphate and not phosphatidylinositol 3,4-bisphosphate is the probable precursor of inositol 1,3,4-trisphosphate in agonist-stimulated parotid gland.
MedLine Citation:
PMID:  2432882     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When [3H]inositol-prelabelled rat parotid-gland slices were stimulated with carbachol, noradrenaline or Substance P, the major inositol trisphosphate produced with prolonged exposure to agonists was, in each case, inositol 1,3,4-trisphosphate. Much lower amounts of radioactivity were present in the inositol 1,4,5-trisphosphate fraction separated by anion-exchange h.p.l.c. Analysis of the inositol trisphosphate head group of phosphatidylinositol bisphosphate in [32P]Pi-labelled parotid glands showed the presence of phosphatidylinositol 4,5-bisphosphate, but no detectable phosphatidylinositol 3,4-bisphosphate. Carbachol-stimulated [3H]inositol-labelled parotid glands contained an inositol polyphosphate with the chromatographic properties and electrophoretic mobility of an inositol tetrakisphosphate, the probable structure of which was determined to be inositol 1,3,4,5-tetrakisphosphate. Since an enzyme in erythrocyte membranes is capable of degrading this tetrakisphosphate to inositol 1,3,4-trisphosphate, it is suggested to be the precursor of inositol 1,3,4-trisphosphate in parotid glands.
Authors:
C P Downes; P T Hawkins; R F Irvine
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  238     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1986 Sep 
Date Detail:
Created Date:  1987-01-29     Completed Date:  1987-01-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  501-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbachol / pharmacology
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Inositol 1,4,5-Trisphosphate
Inositol Phosphates / metabolism*
Norepinephrine / pharmacology
Parotid Gland / drug effects,  metabolism*
Rats
Substance P / pharmacology
Sugar Phosphates / metabolism*
Chemical
Reg. No./Substance:
0/Inositol Phosphates; 0/Sugar Phosphates; 102850-29-3/inositol-1,3,4,5-tetrakisphosphate; 33507-63-0/Substance P; 51-41-2/Norepinephrine; 51-83-2/Carbachol; 85166-31-0/Inositol 1,4,5-Trisphosphate
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