Document Detail

Inorganic thiophosphate effects on chromaffin cell structure and function.
MedLine Citation:
PMID:  1304867     Owner:  NLM     Status:  MEDLINE    
The effect was determined of replacing medium inorganic phosphate with thiophosphate on the structure and function of cultured bovine chromaffin cells. Cell cultures were incubated in normal medium containing fetal bovine serum, phosphate free medium or similar medium supplemented with inorganic phosphate or thiophosphate. In contrast to the other media, cells cultured with thiophosphate medium for 3-4 days showed seriously compromised structure and functions. The cells lost 75% of their catecholamine content and their ability to secrete remaining catecholamines in response to nicotine stimulation. Radiolabelled thiophosphate was rapidly taken up by the cells and, in long-term experiments, was incorporated largely into a 97-121 kDa protein band on SDS-PAGE. Additional minor bands were found to a lesser, variable extent. Transmission electron micrographs of cells treated with thiophosphate showed extensive depletion of chromaffin vesicles and disruption of mitochondria, suggesting that the functional damage noted with these cells could be associated with damage to mitochondria. Analysis of general cell metabolic activity by conversion of the dye (3-[3,4-dimethylthiazol-2-yl]-3,5-diphenyltetrazolium bromide) to its formazan derivative indicated increased metabolic activity at early stages of exposure to thiophosphate followed by a decline with continued exposure, supporting the argument for an overall depression of cell metabolism. Uptake of the dye neutral red, which is avidly accumulated by chromaffin cells, was also reduced for cells exposed to thiophosphate. The data suggest that thiophosphate enters chromaffin cells and disrupts energy dependent cell functions, including catecholamine storage and secretion.
J C Brooks; M H Brooks; S W Carmichael
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemistry international     Volume:  20     ISSN:  0197-0186     ISO Abbreviation:  Neurochem. Int.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1993-07-15     Completed Date:  1993-07-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8006959     Medline TA:  Neurochem Int     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  511-9     Citation Subset:  IM    
Marquette University School of Dentistry, Department of Basic Sciences, Milwaukee, WI 53233.
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MeSH Terms
Catecholamines / antagonists & inhibitors,  metabolism
Cells, Cultured
Chromaffin System / cytology*,  drug effects*,  metabolism
Coloring Agents
Culture Media
Electrophoresis, Polyacrylamide Gel
Microscopy, Electron
Neutral Red
Nicotine / pharmacology
Osmolar Concentration
Phosphates / pharmacology*
Tetrazolium Salts
Grant Support
1 R15 NS23101-01A2/NS/NINDS NIH HHS
Reg. No./Substance:
0/Catecholamines; 0/Coloring Agents; 0/Culture Media; 0/Phosphates; 0/Tetrazolium Salts; 0/Thiazoles; 13598-51-1/thiophosphoric acid; 298-93-1/thiazolyl blue; 54-11-5/Nicotine; 553-24-2/Neutral Red

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