Document Detail

Inoculum size-dependent interactive regulation of metabolism and stress response of Saccharomyces cerevisiae revealed by comparative metabolomics.
MedLine Citation:
PMID:  19808067     Owner:  NLM     Status:  MEDLINE    
To investigate the metabolic regulation against inoculum density and stress response to high cell density, comparative metabolomic analysis was employed on Saccharomyces cerevisiae under fermentations with five different inoculum sizes by gas chromatography time-of-flight mass spectrometry. Samples from these fermentations were clearly distinguished by principal components analysis, indicating that inoculum size had a profound effect on the metabolism of S. cerevisiae. Potential biomarkers responsible for the discrimination were identified as glycerol, phosphoric acid, succinate, glycine, isoleucine, proline, palmitoleic acid, myo-inositol and ethanolamine. It indicated that enhanced stress protectants in glycerol biosynthesis and amino acid metabolism, depressed citric acid cycle intermediates, as well as decreased metabolites relating to membrane structure and function were involved as the inoculum size of yeast increased. Furthermore, significantly higher levels of glycerol and proline in yeast cells of higher inoculum size fermentation (40 g l(-1)) revealed that they played important roles in protecting yeast from stresses in high cell density fermentation. These findings provided new insights into characterizing the metabolic regulation and stress response depending on inoculum density during ethanol fermentation.
Ming-Zhu Ding; Hong-Chi Tian; Jing-Sheng Cheng; Ying-Jin Yuan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-04
Journal Detail:
Title:  Journal of biotechnology     Volume:  144     ISSN:  1873-4863     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-27     Completed Date:  2010-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  279-86     Citation Subset:  IM    
Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering & Technology, Tianjin University, Nankai District, Tianjin 300072, PR China.
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MeSH Terms
Culture Media
Ethanol / metabolism
Gas Chromatography-Mass Spectrometry
Gene Expression Regulation, Fungal*
Heat-Shock Response*
Industrial Microbiology / methods
Saccharomyces cerevisiae* / genetics,  growth & development,  metabolism,  physiology
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Reg. No./Substance:
0/Culture Media; 0/Saccharomyces cerevisiae Proteins; 64-17-5/Ethanol

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