Document Detail

Inoculation and virulence assay for bacterial blight and bacterial leaf streak of rice.
MedLine Citation:
PMID:  23135857     Owner:  NLM     Status:  In-Data-Review    
Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc) cause bacterial blight and bacterial leaf streak in rice, respectively. Despite being very closely related, the pathogens colonize different tissues and cause distinct diseases. The diseases are economically important and also serve as model systems for studying plant-bacterial interactions. Here we describe protocols for Xoo and Xoc inoculation and disease scoring methods that are appropriate to their different modes of infection. These methods are routinely used to evaluate pathogen virulence or host responses under controlled environmental conditions.
Bing Yang; Adam Bogdanove
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  956     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  249-55     Citation Subset:  IM    
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA,
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