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Innovative approaches for effective selection of lipase-producing microorganisms as whole cell catalysts for biodiesel production.
MedLine Citation:
PMID:  21458602     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The high cost of commercial lipases limits their industrial application in the production of biodiesel or fatty acid methyl esters (FAME). This disadvantage has encouraged the search of lipase-producing microorganisms (LPM) as potential whole cell catalysts for FAME production. The aim of this study, therefore, was to evaluate innovative procedures for easy selection and testing of lipase-producing microorganisms (LPM) as a low-cost whole cell catalyst, based on catalytic performance, methanol tolerance, and physico-chemical cell surface properties. The latter (in particular the cell surface hydrophobicity and charge) were analyzed because of their crucial role in microbial adhesion to surfaces and the concomitant increase in cell immobilization and bioavailability of hydrophobic substrates. Biocatalysis experiments performed in the presence of nutrient, rapeseed oil and methanol was an effective tool for studying and identifying in just two experiments the capacity of different LPMs as biocatalysts in organic media, as well the methanol tolerance of the cell and the lipase. This indicates the potential for using live microorganisms for FAME production. Another finding was that the inhibitory effect of methanol is more significant for lipase activity than LPM growth, indicating that the way in which alcohol is supplied to the reaction is a critical step in FAME production by biocatalysts. According to these results, the application of these innovative assessments should simplify the search for new strains which are able to effectively catalyze the FAME production process.
Authors:
Gustavo Ciudad; Isaac Reyes; Laura Azócar; Reinaldo Briones; Milko Jorquera; Lukas Y Wick; Rodrigo Navia
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-30
Journal Detail:
Title:  New biotechnology     Volume:  -     ISSN:  1876-4347     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-4-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101465345     Medline TA:  N Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier B.V.
Affiliation:
Scientific and Technological Bioresources Nucleus, Universidad de La Frontera, Casilla 54-D, Temuco, Chile.
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