Document Detail

Innocuous intracellular ice improves survival of frozen cells.
MedLine Citation:
PMID:  12428746     Owner:  NLM     Status:  MEDLINE    
Extensive efforts to avoid intracellular ice formation (IF) during freezing have been central to current methods used for the preservation and long-term storage of cells and tissues. In this study, we examined the effect of intracellular ice formation on the postthaw survival of V-79W fibroblast and MDCK epithelial cells using convection cryomicroscopy and controlled-rate freezing. V-79W and MDCK cells were cultured as single attached cells or as confluent cell monolayers. Postthaw cell survival was assessed using three different indices: the presence of an intact plasma membrane, the ability to reduce alamarBlue, and the capacity to form colonies in culture. Regulating the isothermal nucleation temperature was used to control the incidence of IIF in the model systems. We report that the presence of intracellular ice in confluent monolayers at high subzero temperatures does not adversely affect postthaw cell survival. Further, we show that in the absence of chemical cryoprotectants, the formation of intracellular ice alone improves the postthaw survival of cultured V-79W fibroblast and MDCK epithelial cells. Improved long-term storage of cells and tissues will result by incorporating innocuous intracellular ice formation into current strategies for cryopreservation.
Jason P Acker; Locksley E McGann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell transplantation     Volume:  11     ISSN:  0963-6897     ISO Abbreviation:  Cell Transplant     Publication Date:  2002  
Date Detail:
Created Date:  2002-11-13     Completed Date:  2003-04-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  563-71     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Membrane / physiology
Cell Membrane Permeability
Cell Survival / drug effects,  physiology*
Cryopreservation / methods*
Cryoprotective Agents / pharmacology
Dimethyl Sulfoxide / pharmacology*
Reg. No./Substance:
0/Cryoprotective Agents; 0/Ice; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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