| Innate inflammatory responses of human decidual cells to periodontopathic bacteria. | |
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MedLine Citation:
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PMID: 20452492 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The purpose of this study was to test the hypothesis that periodontopathic bacteria exert potent proinflammatory effects in human decidua. STUDY DESIGN: The immunostimulatory effects of Gram-positive and negative periodontopathic bacteria and their lipopolysaccharides were tested in human decidual cell cultures in comparison with Escherichia coli. Cytokine production was measured by enzyme-linked immunosorbent assay; inflammatory gene expression was measured by oligonucleotide arrays and quantitative real time-polymerase chain reaction. RESULTS: All bacteria that were tested elicited an inflammatory response, although concentration-dependence and efficacy varied considerably with organism and culture. Lipopolysaccharides were more potent stimuli than intact bacterial cells, although bacteria exerted greater effects at high concentrations. Of 112 genes on the arrays, 18 genes were stimulated significantly by one or more lipopolysaccharide preparation. CONCLUSION: The ability of periodontopathic bacteria to stimulate a decidual inflammatory response is highly variable and partly dependent on the presence and structure of constituent lipopolysaccharides. This adds to our understanding of the causal association between periodontal disease and preterm birth. |
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Authors:
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Jeffrey A Keelan; Pui-Mun Wong; Philip S Bird; Murray D Mitchell |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of obstetrics and gynecology Volume: 202 ISSN: 1097-6868 ISO Abbreviation: Am. J. Obstet. Gynecol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-10 Completed Date: 2010-06-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370476 Medline TA: Am J Obstet Gynecol Country: United States |
Other Details:
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Languages: eng Pagination: 471.e1-11 Citation Subset: AIM; IM |
Copyright Information:
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Crown Copyright (c) 2010. Published by Mosby, Inc. All rights reserved. |
Affiliation:
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School of Women's and Infants' Health, University of Western Australia, Perth, WA, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actinobacteria
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immunology Cells, Cultured Chemokine CCL3 / metabolism Decidua / cytology*, immunology, microbiology* Dental Plaque / microbiology Escherichia coli Female Fusobacterium nucleatum / immunology Gene Expression Profiling Humans Inflammation / microbiology Interleukin-6 / metabolism Lipopolysaccharides / pharmacology Oligonucleotide Array Sequence Analysis Peptostreptococcus / immunology Periodontal Diseases / epidemiology Porphyromonas gingivalis / immunology Premature Birth / epidemiology, microbiology Tumor Necrosis Factor-alpha / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CCL3; 0/Interleukin-6; 0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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