| Injury to cultured endothelial cells by thrombin-stimulated platelets. | |
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MedLine Citation:
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PMID: 3959543 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In vivo, stimulated platelets may injure the endothelium. We have used cultured endothelial cells to assess endothelial cell damage caused by platelet stimulation with thrombin. Endothelial cells were cultured from umbilical veins and semiconfluent cultures were labeled with Na2 51CrO4. Twenty four hours later washed human platelets (final concentration 200,000 platelets/microliters) and thrombin (final concentration 4 units/ml) were added to the medium and the culture dish was shaken for 15 minutes. The percentage of cells detached from the culture dish and the percentage of 51Cr lost from the endothelial cells into the ambient fluid during the shaking were determined and used as indicators of cell injury. Increased percentages of loosened cells and 51Cr in the ambient fluid were observed with platelet suspension and thrombin compared to controls with neither platelet suspension nor thrombin and controls with either platelet suspension or thrombin. The platelet-free supernatant obtained after reaction of the platelets with thrombin also increased the percentage of loosened cells, but it did not increase the percentage of 51Cr in the ambient fluid to a significant degree. Thrombin alone caused a moderate loss of 51Cr, but no increased loosening of cells. Treatment of the platelets with acetylsalicylic acid prior to the experiment depressed the detachment effect of thrombin-stimulated platelets, but did not alter the effect on the release of 51Cr into the ambient fluid. Scanning and transmission electron microscopy of cultured endothelial cells exposed to thrombin-stimulated platelets confirmed the presence of loosening and injury to the endothelial cells. Thus, platelet stimulation with thrombin had at least two effects on the cultured endothelial cells: a loosening effect caused by material released from the platelets; an injury effect which, in order to reach its maximum, required the presence of stimulated platelets. |
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Authors:
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L Jørgensen; A G Grøthe; T Larsen; R L Kinlough-Rathbone; J F Mustard |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Laboratory investigation; a journal of technical methods and pathology Volume: 54 ISSN: 0023-6837 ISO Abbreviation: Lab. Invest. Publication Date: 1986 Apr |
Date Detail:
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Created Date: 1986-05-05 Completed Date: 1986-05-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376617 Medline TA: Lab Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 408-15 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspirin
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pharmacology Blood Platelets / drug effects, physiology* Blood Vessels / pathology*, ultrastructure Cells, Cultured Chromium Radioisotopes / diagnostic use Endothelium / pathology, ultrastructure Humans Microscopy, Electron Microscopy, Electron, Scanning Platelet Adhesiveness Thrombin / physiology* Time Factors Umbilical Veins |
| Chemical | |
Reg. No./Substance:
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0/Chromium Radioisotopes; 50-78-2/Aspirin; EC 3.4.21.5/Thrombin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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