Document Detail

Injury to axons and oligodendrocytes following endothelin-1-induced middle cerebral artery occlusion in conscious rats.
MedLine Citation:
PMID:  16905121     Owner:  NLM     Status:  MEDLINE    
Injury to axons and oligodendrocytes has been poorly characterized in most animal models of stroke, and hence has been difficult to target therapeutically. It is therefore necessary to characterize axonal and oligodendroglial injury in these models, in order to rationally design putative protective compounds that minimize this injury. This study aims to characterize injury to axons and oligodendrocytes in the endothelin-1 (ET-1) model of middle cerebral artery occlusion (MCAO) in conscious rats. Transient forebrain ischemia was induced in conscious adult male Long Evans rats by the perivascular microinjection of ET-1. Quantitative histopathology was performed on forebrain sections at 6, 24, 48 and 72 h after ET-1 administration, using ballistic light analyses and immunohistochemistry for amyloid precursor protein (APP), SMI32, and Tau-1. Ballistic light analyses of cortical and striatal lesions revealed that the infarct volume was maximal in these regions by 6 h. APP and SMI32 immunohistochemistry demonstrated that axonal injury was maximal by 6 h in this model; however, some injured axons appeared to maintain good structural integrity up to 72 h after insult. Density measurements for Tau-1-immunopositive oligodendrocytes were significantly elevated within the corpus callosum from 48 h, but reductions in total oligodendrocyte numbers were not apparent up 72 h after ET-1 injection. These results indicate that axonal and oligodendroglial injury should be investigated as potential targets for delayed therapeutic intervention after MCAO.
Melissa M Gresle; Bevyn Jarrott; Nicole M Jones; Jennifer K Callaway
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-14
Journal Detail:
Title:  Brain research     Volume:  1110     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-18     Completed Date:  2006-12-06     Revised Date:  2011-08-02    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  13-22     Citation Subset:  IM    
Howard Florey Institute, Brain Injury and Repair Program, University of Melbourne, Parkville, Australia.
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MeSH Terms
Amyloid beta-Protein Precursor / metabolism
Antibodies, Monoclonal / metabolism
Brain / metabolism,  pathology
Cell Count / methods
Diffuse Axonal Injury / etiology,  pathology*
Disease Models, Animal
Immunohistochemistry / methods
Infarction, Middle Cerebral Artery / chemically induced*,  pathology,  physiopathology
Neurofilament Proteins / metabolism
Oligodendroglia / pathology*
Rats, Long-Evans
Time Factors
Reg. No./Substance:
0/Amyloid beta-Protein Precursor; 0/Antibodies, Monoclonal; 0/Endothelin-1; 0/Neurofilament Proteins; 0/tau-1 monoclonal antibody

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