| Injured kidney cells express SM22α (transgelin): Unique features distinct from α-smooth muscle actin (αSMA). | |
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MedLine Citation:
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PMID: 21272134 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: SM22α (transgelin) has been focused upon as a player in the process of phenotypic changes of types of cells. The SM22α expression in the rat anti-glomerular basement membrane (GBM) nephritis model and differences from an established phenotypic marker for the myofibroblast, α-smooth muscle actin (αSMA), were investigated. METHODS: The rat kidney tissues were processed for histological studies, immunohistochemical and immunoelectronmicroscopy analyses on days 0, 7, 28, 42 and 56 after injection of rabbit anti-GBM serum for the disease induction. RESULTS: Immunohistochemistry with anti-SM22α antibodies (Ab) revealed that kidneys of the nephritic rats on day 7 expressed SM22α in podocytes, crescentic cells and epithelial cells of Bowman's capsule. After 28 days, SM22α was also expressed in peritubular interstitial cells. Double immunofluorescence with anti-SM22α Ab and anti-αSMA Ab showed that SM22α was preferentially expressed in podocytes, whereas αSMA was positive in mesangial cells on day 7. After day 28, both molecules became positive in peritubular interstitial cells. CONCLUSION: SM22α was expressed in epithelial cells of inflamed glomeruli in the early phase, and then also in peritubular interstitial cells in the later phase of anti-GBM nephritis model. SM22α presented unique kinetics of expression distinct from αSMA. |
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Authors:
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Yuichi Sakamaki; Minoru Sakatsume; Xingzhi Wang; Shigeru Inomata; Tadashi Yamamoto; Fumitake Gejyo; Ichiei Narita |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nephrology (Carlton, Vic.) Volume: 16 ISSN: 1440-1797 ISO Abbreviation: Nephrology (Carlton) Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-28 Completed Date: 2011-06-07 Revised Date: 2011-11-10 |
Medline Journal Info:
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Nlm Unique ID: 9615568 Medline TA: Nephrology (Carlton) Country: Australia |
Other Details:
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Languages: eng Pagination: 211-8 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Nephrology © 2010 Asian Pacific Society of Nephrology. |
Affiliation:
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Division of Clinical Nephrology and Rheumatology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism* Animals Basement Membrane / immunology* Biological Markers / metabolism Disease Models, Animal Epithelial Cells / metabolism Glomerulonephritis / chemically induced, metabolism*, pathology Immunoglobulin G Kidney Glomerulus / metabolism*, pathology Male Microfilament Proteins / metabolism* Muscle Proteins / metabolism* Podocytes / metabolism Rats Rats, Inbred WKY |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Biological Markers; 0/Immunoglobulin G; 0/Microfilament Proteins; 0/Muscle Proteins; 0/smooth muscle actin, rat; 0/transgelin |
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