Document Detail

Injections of recombinant human erythropoietin increases lactate influx into erythrocytes.
MedLine Citation:
PMID:  14966016     Owner:  NLM     Status:  MEDLINE    
Previous studies showed that erythropoietin not only increases erythrocyte production but is also essential in both the synthesis and the good functioning of several erythrocyte membrane proteins, including band 3. It is still unknown whether anion and/or H(+) fluxes are modified by erythropoietin. This study aimed to evaluate the effect of recombinant human erythropoietin (rHuEPO) injections on lactate transport into erythrocytes via band 3 and H(+)-monocarboxylate transporter MCT-1, two proteins involved in lactate exchange. Nine athletes received subcutaneous rHuEPO (50 U/kg body mass 3 times a week for 4 wk), and seven athletes received a saline solution (placebo group). All subjects were also supplemented with oral iron and vitamins B(9) and B(12). Lactate transport into erythrocytes was studied before and after the rHuEPO treatment at different lactate concentrations (1.6, 8.1, 41, and 81.1 mM). After treatment, MCT-1 lactate uptake was increased at 1.6, 41 (P < 0.01), and 81.1 mM lactate concentration (P < 0.001) although lactate uptake via band 3 and nonionic diffusion were unchanged. MCT-1 maximal velocity increased in the rHuEPO group (P < 0.05), reaching higher values than in the placebo group (P < 0.05) after treatment. Our results show that rHuEPO injections increased MCT-1 lactate influx at low and high lactate concentrations. The increase in MCT-1 maximal velocity suggests that rHuEPO may stimulate MCT-1 synthesis during erythrocyte formation in bone marrow.
Philippe Connes; Corinne Caillaud; Jacques Mercier; Didier Bouix; Jean François Casties
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Publication Detail:
Type:  Clinical Trial; Journal Article     Date:  2004-02-13
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  97     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-28     Completed Date:  2004-12-20     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  326-32     Citation Subset:  IM    
Faculté des Sciences du Sport et de l'Education Physique, F-34090 Montpellier, France.
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MeSH Terms
Anaerobic Threshold / physiology
Bicycling / physiology
Erythrocytes / drug effects,  metabolism*
Erythropoietin / administration & dosage,  pharmacology*
Exercise Test
Hemoglobins / metabolism
Lactic Acid / blood*
Monocarboxylic Acid Transporters / metabolism
Oxygen Consumption / physiology
Physical Endurance / physiology
Recombinant Proteins
Reticulocytes / metabolism
Running / physiology
Swimming / physiology
Symporters / metabolism
Reg. No./Substance:
0/Hemoglobins; 0/Monocarboxylic Acid Transporters; 0/Recombinant Proteins; 0/Symporters; 0/monocarboxylate transport protein 1; 11096-26-7/Erythropoietin; 50-21-5/Lactic Acid

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