Document Detail

Injection of chemoattractants into normal cornea: a model of inflammation after alkali injury.
MedLine Citation:
PMID:  9699566     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The objective of this study was to establish and characterize the invasion of polymorphonuclear leukocytes (PMNs) into a normal cornea after intrastromal injection of the tripeptide chemoattractants generated from alkali-degraded corneas. METHODS: The following samples were injected into the midstroma of normal rabbit corneas: ultrafiltered tripeptide chemoattractants (N-acetyl-proline-glycine-proline and N-methyl-proline-glycine-proline) generated from alkali-degraded corneas, synthetic N-acetyl-PGP, positive control (leukotriene B4 [LTB4]), or negative control (Hanks' balanced salt solution [HBSS]). Timed responses of PMN infiltration were established for effective concentrations of LTB4 or the ultrafiltered chemoattractants. RESULTS: All intrastromal injections resulted in the immediate development of an edematous disc that was 10 mm in diameter. The lesion essentially had cleared in the HBSS-injected eyes by 8 hours, and histologic sections revealed minimal numbers of PMNs in the cornea or limbal tissue. The injection of LTB4 or the ultrafiltered tripeptide chemoattractants induced peak numbers of PMNs within the stroma at 8 hours, subsiding by 16 hours. Seventy units of ultrafiltered chemoattractants yielded a strong PMN response, similar to 1 X 10(-5) M LTB4. The highest concentration of ultrafiltered chemoattractants (350 U) produced a severe PMN response that was characterized by a solid sheet of neutrophils surrounding the injection site. The injection of synthetic N-acetyl-PGP (2 X 10(-4) M) produced a marked PMN response. CONCLUSIONS: PMN invasion of the normal cornea after the injection of the ultrafiltered tripeptide chemoattractants or the synthetic N-acetyl-PGP mimicked early PMN infiltration in the alkali-injured eye, confirming the importance of this chemoattractant as an inflammatory mediator.
R R Pfister; J L Haddox; C I Sommers
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  39     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-08-13     Completed Date:  1998-08-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1744-50     Citation Subset:  IM    
The Eye Research Laboratories, Brookwood Medical Center, Birmingham, Alabama, USA.
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MeSH Terms
Burns, Chemical / immunology,  pathology
Chemotactic Factors / pharmacology*
Chemotaxis, Leukocyte*
Corneal Stroma / drug effects*,  immunology,  pathology
Disease Models, Animal*
Eye Burns / chemically induced,  pathology
Keratitis / immunology*,  pathology
Leukocyte Count
Leukotriene B4 / pharmacology
Neutrophils / immunology*,  pathology
Oligopeptides / pharmacology
Sodium Hydroxide / adverse effects
Grant Support
Reg. No./Substance:
0/Chemotactic Factors; 0/Oligopeptides; 1310-73-2/Sodium Hydroxide; 71160-24-2/Leukotriene B4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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