Document Detail


Injection by hydrostatic pressure in conjunction with electrokinetic force on a microfluidic chip.
MedLine Citation:
PMID:  15213989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple method was developed for injecting a sample on a cross-form microfluidic chip by means of hydrostatic pressure combined with electrokinetic forces. The hydrostatic pressure was generated simply by adjusting the liquid level in different reservoirs without any additional driven equipment such as a pump. Two dispensing strategies using a floating injection and a gated injection, coupled with hydrostatic pressure loading, were tested. The fluorescence observation verified the feasibility of hydrostatic pressure loading in the separation of a mixture of fluorescein sodium salt and fluorescein isothiocyanate. This method was proved to be effective in leading cells to a separation channel for single cell analysis.
Authors:
Hongwei Gai; Linfen Yu; Zhongpeng Dai; Yinfa Ma; Bingcheng Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  25     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-23     Completed Date:  2005-01-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1888-94     Citation Subset:  IM    
Affiliation:
Dalian Institute of Chemical Physics, CAS, Dalian, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carps / blood*
Electromagnetic Fields*
Electrophoresis, Microchip*
Erythrocytes / cytology*
Fluorescence
Hydrostatic Pressure
Microfluidics*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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