Document Detail


Injectable microsphere/hydrogel hybrid system containing heat shock protein as therapy in a murine myocardial infarction model.
MedLine Citation:
PMID:  23952941     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(.,.-lactic-co-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks in vitro. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated in vivo. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.
Authors:
Jangwook Lee; Min-Ji Cha; Kwang Suk Lim; Jang-Kyung Kim; Sang-Kyung Lee; Yong-Hee Kim; Ki-Chul Hwang; Kuen Yong Lee
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-8-19
Journal Detail:
Title:  Journal of drug targeting     Volume:  -     ISSN:  1029-2330     ISO Abbreviation:  J Drug Target     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-8-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312476     Medline TA:  J Drug Target     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Bioengineering, Hanyang University , Seoul , Republic of Korea .
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