Document Detail


Initiation of swarming motility by Proteus mirabilis occurs in response to specific cues present in urine and requires excess L-glutamine.
MedLine Citation:
PMID:  23316040     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proteus mirabilis, a leading cause of catheter-associated urinary tract infection (CaUTI), differentiates into swarm cells that migrate across catheter surfaces and medium solidified with 1.5% agar. While many genes and nutrient requirements involved in the swarming process have been identified, few studies have addressed the signals that promote initiation of swarming following initial contact with a surface. In this study, we show that P. mirabilis CaUTI isolates initiate swarming in response to specific nutrients and environmental cues. Thirty-three compounds, including amino acids, polyamines, fatty acids, and tricarboxylic acid (TCA) cycle intermediates, were tested for the ability to promote swarming when added to normally nonpermissive media. L-Arginine, L-glutamine, DL-histidine, malate, and DL-ornithine promoted swarming on several types of media without enhancing swimming motility or growth rate. Testing of isogenic mutants revealed that swarming in response to the cues required putrescine biosynthesis and pathways involved in amino acid metabolism. Furthermore, excess glutamine was found to be a strict requirement for swarming on normal swarm agar in addition to being a swarming cue under normally nonpermissive conditions. We thus conclude that initiation of swarming occurs in response to specific cues and that manipulating concentrations of key nutrient cues can signal whether or not a particular environment is permissive for swarming.
Authors:
Chelsie E Armbruster; Steven A Hodges; Harry L T Mobley
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-01-11
Journal Detail:
Title:  Journal of bacteriology     Volume:  195     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-25     Completed Date:  2013-04-17     Revised Date:  2014-11-09    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1305-19     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Catheter-Related Infections / microbiology
Female
Glutamine / metabolism*
Humans
Movement
Proteus Infections / microbiology
Proteus mirabilis / genetics,  metabolism,  physiology*
Putrescine / biosynthesis
Signal Transduction
Urea / metabolism
Urinary Catheterization / adverse effects
Urinary Tract Infections / microbiology
Urine / chemistry*
Grant Support
ID/Acronym/Agency:
DK089503/DK/NIDDK NIH HHS; F32 AI102552/AI/NIAID NIH HHS; F32AI102552/AI/NIAID NIH HHS; R01AI059722/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0RH81L854J/Glutamine; 8W8T17847W/Urea; V10TVZ52E4/Putrescine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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