| Initiation of protein synthesis in bacteria at a translational start codon of mamalian cDNA: effects of the preceding nucleotide sequence. | |
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MedLine Citation:
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PMID: 6154933 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasmids containing a mouse cDNA sequence encoding the enzyme dihydrofolate reductase (DHFR; tetrahydrofolate dehydrogenase; 5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3) have been used to study the efficiency of initiation of protein synthesis at an ATG (AUG) translational start codon indigenous to the eukaryotic CDNA. differences in DHFR production assayed phenotypically, enzymatically, and immunologically were correlated with the primary structure of the DNA segment that precedes the translational start codon. Our results indicate that initiation of a structurally discrete and biologically functional eukaryotic protein can occur in bacteria on a fused mRNA molecule, and that the efficiency of expression is strongly affected by: (i) the extent of homology of the translational control region with the 3'-OH end of 16S ribosomal RNA, and (ii) the distance between the protein start codon and the ribosome-binding sequence on the mRNA. |
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Authors:
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A C Chang; H A Erlich; R P Gunsalus; J H Nunberg; R J Kaufman; R T Schimke; S N Cohen |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 77 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1980 Mar |
Date Detail:
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Created Date: 1980-07-22 Completed Date: 1980-07-22 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1442-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Codon DNA, Recombinant* Epitopes Escherichia coli / genetics* Genes* Mice Operon Peptide Chain Initiation, Translational* RNA, Messenger / genetics Tetrahydrofolate Dehydrogenase / genetics*, immunology beta-Lactamases / genetics |
| Chemical | |
Reg. No./Substance:
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0/Codon; 0/DNA, Recombinant; 0/Epitopes; 0/RNA, Messenger; EC 1.5.1.3/Tetrahydrofolate Dehydrogenase; EC 3.5.2.6/beta-Lactamases |
| Comments/Corrections | |
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