Document Detail


Initiation and maintenance of in vitro decidualization are independent of hormonal sensitization in vivo.
MedLine Citation:
PMID:  1477205     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of in vivo hormonal sensitization on the competence of uterine stromal (US) cells to decidualize in vitro were assessed. In vitro differentiation of uterine stroma isolated from Day 4 pregnant rats, sensitized to respond to a decidual stimulus, was compared to that in nonsensitized immature, castrated or cycling rats. The initiation of in vitro decidualization--as monitored by the expression of the decidual markers desmin and laminin in rat US cells--was independent of the hormonal status of the animal from which the cells were isolated and occurred in the absence of serum in the medium. Differentiation was accelerated in high-density cultures where contact inhibition suppressed proliferation and decreased the extent of cell growth. The extent to which in vitro decidualization imitates in vivo stromal cell differentiation was assessed by comparing decidualization in the rabbit, a species with only a limited decidual cell response, and in the rat. US cells isolated from nonpregnant rabbits differentiated in vitro by expressing laminin, but not desmin. Indirect immunofluorescence of frozen uterine sections from pregnant and nonpregnant rabbits validated in vitro differentiation as a faithful reflection of the in vivo program of decidualization. Although the program of US cell differentiation may vary between the species, initiation of differentiation in vitro appeared to be independent of hormonal preparation in vivo for both the species examined.
Authors:
S K Mani; J Julian; S Lampelo; S R Glasser
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  47     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1993-02-09     Completed Date:  1993-02-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  785-99     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Cell Communication
Cell Differentiation / physiology
Cell Division / physiology
Decidua / growth & development*,  metabolism
Desmin / biosynthesis
Electrophoresis, Polyacrylamide Gel
Female
Gonadal Steroid Hormones / physiology*
Laminin / biosynthesis
Microscopy, Fluorescence
Pregnancy
Rabbits
Rats
Rats, Sprague-Dawley
Stromal Cells / physiology
Uterus / cytology*
Grant Support
ID/Acronym/Agency:
HD-07495/HD/NICHD NIH HHS; HD-22785/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Desmin; 0/Gonadal Steroid Hormones; 0/Laminin

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