| Initiation and maintenance of in vitro decidualization are independent of hormonal sensitization in vivo. | |
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MedLine Citation:
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PMID: 1477205 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of in vivo hormonal sensitization on the competence of uterine stromal (US) cells to decidualize in vitro were assessed. In vitro differentiation of uterine stroma isolated from Day 4 pregnant rats, sensitized to respond to a decidual stimulus, was compared to that in nonsensitized immature, castrated or cycling rats. The initiation of in vitro decidualization--as monitored by the expression of the decidual markers desmin and laminin in rat US cells--was independent of the hormonal status of the animal from which the cells were isolated and occurred in the absence of serum in the medium. Differentiation was accelerated in high-density cultures where contact inhibition suppressed proliferation and decreased the extent of cell growth. The extent to which in vitro decidualization imitates in vivo stromal cell differentiation was assessed by comparing decidualization in the rabbit, a species with only a limited decidual cell response, and in the rat. US cells isolated from nonpregnant rabbits differentiated in vitro by expressing laminin, but not desmin. Indirect immunofluorescence of frozen uterine sections from pregnant and nonpregnant rabbits validated in vitro differentiation as a faithful reflection of the in vivo program of decidualization. Although the program of US cell differentiation may vary between the species, initiation of differentiation in vitro appeared to be independent of hormonal preparation in vivo for both the species examined. |
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Authors:
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S K Mani; J Julian; S Lampelo; S R Glasser |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biology of reproduction Volume: 47 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1993-02-09 Completed Date: 1993-02-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 785-99 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Cell Communication Cell Differentiation / physiology Cell Division / physiology Decidua / growth & development*, metabolism Desmin / biosynthesis Electrophoresis, Polyacrylamide Gel Female Gonadal Steroid Hormones / physiology* Laminin / biosynthesis Microscopy, Fluorescence Pregnancy Rabbits Rats Rats, Sprague-Dawley Stromal Cells / physiology Uterus / cytology* |
| Grant Support | |
ID/Acronym/Agency:
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HD-07495/HD/NICHD NIH HHS; HD-22785/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Desmin; 0/Gonadal Steroid Hormones; 0/Laminin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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