| Initiation of hepatitis delta virus (HDV) replication: HDV RNA encoding the large delta antigen cannot replicate. | |
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MedLine Citation:
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PMID: 12237434 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The hepatitis delta virus (HDV) nucleocapsid consists of a genomic-length RNA of 1.7 kb and approximately equimolar amounts of the small and large forms of the hepatitis delta antigen (S-HDAg and L-HDAg, respectively). Since HDV RNA particles contain not only a genomic RNA species encoding S-HDAg but also an RNA species encoding L-HDAg, which is produced by an RNA-editing process, the question arises as to whether RNAs encoding either L-HDAg or S-HDAg can initiate replication. To study this, two cDNA-free transfection methods were employed: HDV RNA cotransfected with either the S-HDAg-encoding mRNA species or the ribonucleocapsid protein complex, comprising HDV RNA and recombinant S-HDAg. Results showed that the genomic-sense RNA encoding S-HDAg could promote HDV replication, whereas the L-HDAg-encoding RNA species was unable to replicate under the same conditions. The antigenomic RNA species encoding either S-HDAg or L-HDAg could not replicate by either of these procedures. In addition, L-HDAg alone could not promote replication of the genomic RNA but, by supplementing an equal amount of S-HDAg, replication occurred. These data indicate that L-HDAg-encoding RNA species are probably not involved in the initiation of HDV RNA synthesis; instead, their main function may be to serve as template for producing L-HDAg, which regulates HDV RNA synthesis and virion assembly. These results suggest that the genomic RNA species encoding S-HDAg is the only functional genome for HDV infection and explain why the presence of the edited HDV RNA encoding L-HDAg does not interfere with HDV infection. |
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Authors:
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Gwo-Tarng Sheu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of general virology Volume: 83 ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2002-09-18 Completed Date: 2002-10-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: England |
Other Details:
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Languages: eng Pagination: 2507-13 Citation Subset: IM |
Affiliation:
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Institute of Toxicology, Chung Shan Medical University, 110 Sec. 1, Chien-Kuo N. Road, Taichung 40203, Taiwan, Republic of China. gtsheu@csmu.edu.tw |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals COS Cells Cercopithecus aethiops Hepatitis Antigens / genetics*, metabolism Hepatitis Delta Virus / genetics*, physiology Hepatitis delta Antigens Mutagenesis, Site-Directed RNA, Viral / biosynthesis* RNA-Binding Proteins / genetics*, metabolism Templates, Genetic Transfection Virus Replication / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Hepatitis Antigens; 0/Hepatitis delta Antigens; 0/RNA, Viral; 0/RNA-Binding Proteins; 0/hepatitis delta virus large antigen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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