Document Detail

Initial stage of transformation of permissive cells by simian virus 40: development of resistance to productive infection.
MedLine Citation:
PMID:  4105906     Owner:  NLM     Status:  MEDLINE    
A quantitative assay has been used to determine the conditions leading to acquisition of resistance of permissive cells to lytic infection. The number of cell colonies surviving infection depends on the occurrence of several cell divisions after infection. High yields of resistant colonies were obtained when infected, confluent cultures were released from contact inhibition 10 to 14 hr after infection. Infection of actively growing cells produced similar results, but halting further division by seeding these growing cells on confluent monolayers prevented the development of colonies. Colony formation was a direct function of multiplicities lower than 5. An inverse killing response was observed with higher multiplicities, yet colonies were produced at a multiplicity of infection as high as 50. Brief exposure of input simian virus 40 to ultraviolet light stimulated colony formation. Irradiation of the virus for longer periods of time led to reduction of colony formation at a rate slower than the rate of inactivation of viral infectivity. It was concluded that resistance is induced by simian virus 40 and that this alteration represents one of the earliest detectable characteristics of the transformation of permissive cells.
E C Hahn; G Sauer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  8     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1971 Jul 
Date Detail:
Created Date:  1971-11-16     Completed Date:  1971-11-16     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7-16     Citation Subset:  IM    
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MeSH Terms
Antigens / analysis
Cell Division
Cell Line
Cell Survival
Cell Transformation, Neoplastic*
Culture Techniques*
DNA / biosynthesis
Fluorescent Antibody Technique
Immune Sera
Radiation Effects
Simian virus 40 / growth & development,  immunology,  pathogenicity*,  radiation effects
Staining and Labeling
Thymidine / metabolism
Time Factors
Ultraviolet Rays
Virus Replication
Reg. No./Substance:
0/Antigens; 0/Immune Sera; 10028-17-8/Tritium; 50-89-5/Thymidine; 9007-49-2/DNA; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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