Document Detail

Initial clonal expansion of germinal center B cells takes place at the perimeter of follicles.
MedLine Citation:
PMID:  19303334     Owner:  NLM     Status:  MEDLINE    
Current models of the germinal center (GC) response propose that after stimulation at the edges of T cell zones, pre-GC B cells directly migrate to the center of follicles and proliferate to form GCs. We followed the interrelationship of proliferation, differentiation, and microenvironmental locale in populations of pre-GC B cells responding to antigen. In contrast to the predictions of current models, after accumulation at the T-B interface, these cells appeared at the perimeter of follicles adjacent to the marginal zone. There, they rapidly proliferated for several days but underwent no V gene hypermutation and little heavy-chain class switching. Their chemokine receptor expression pattern indicated that these cells were sessile, yet they had begun to acquire many phenotypic characteristics of GC B cells. The expanded clones were subsequently observed in the center of follicles, suggesting that GCs are created by coalescence of B cells from this follicular perimeter response.
Francis Coffey; Boris Alabyev; Tim Manser
Related Documents :
8698104 - Apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicl...
2160294 - Regional pattern of cell maturation and progesterone biosynthesis in the avian granulos...
9365194 - Evidence that functional interactions of creb and sf-1 mediate hormone regulated expres...
3115604 - Mechanisms of regulation of rat ovarian 7,12-dimethylbenz[a]anthracene hydroxylase.
12749854 - Monoclonal antibody against dnmt1 arrests the cell division of xenopus early-stage embr...
15326224 - The effect of eukaryotic release factor depletion on translation termination in human c...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-03-19
Journal Detail:
Title:  Immunity     Volume:  30     ISSN:  1097-4180     ISO Abbreviation:  Immunity     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-17     Completed Date:  2009-05-14     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  9432918     Medline TA:  Immunity     Country:  United States    
Other Details:
Languages:  eng     Pagination:  599-609     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
B-Lymphocytes / cytology,  immunology*
Cell Proliferation
Cells, Cultured
Dendritic Cells, Follicular / immunology*
Flow Cytometry
Fluorescent Antibody Technique
Gene Expression Profiling
Germinal Center / cytology*,  immunology*
Mice, Transgenic
Grant Support
R01 AI038965/AI/NIAID NIH HHS; R01 AI038965-12/AI/NIAID NIH HHS; R01 AI046806/AI/NIAID NIH HHS; R01 AI046806-10/AI/NIAID NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Prevalence of diabetes, metabolic syndrome, and cardiovascular risk factors in US Asian Indians: res...
Next Document:  Observation of molecular migration in porous media using 2D exchange spectroscopy in the inhomogeneo...