Document Detail


Initial characterization of viral sequences from a SHIV-inoculated pig-tailed macaque that developed AIDS.
MedLine Citation:
PMID:  8892038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we report on the derivation of a pathogenic SIV-HIV chimeric virus (SHIV) and the initial characterization of the viral sequences from the first (macaque PPc) of a series of pig-tailed macaques that developed CD4+ T cell loss and AIDS. Viral genes were amplified by PCR from the brain, lymphoid, and kidney tissues and their sequences compared to the original SHIV used to initiate passages in macaques. Our results show that the vpu gene, which was nonfunctional in the original SHIV, now coded for functional protein in macaque PPc. The tat and rev genes had no consensus changes but the nef gene had 4-5 consensus changes, depending on the tissue examined. The gp 120 gene had the highest number of nucleotide and amino acid substitution rates that varied from 0.64% to 1.44% and 1.17% to 3.71%, respectively, again depending on the tissue examined. These results suggest that a constellation of changes accumulated at the genomic level during the derivation of a SHIV that was pathogenic for pig-tailed macaques.
Authors:
E B Stephens; S V Joag; D Sheffer; Z Q Liu; L Zhao; S Mukherjee; L Foresman; I Adany; Z Li; D Pinson; O Narayan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of medical primatology     Volume:  25     ISSN:  0047-2565     ISO Abbreviation:  J. Med. Primatol.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1997-02-06     Completed Date:  1997-02-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0320626     Medline TA:  J Med Primatol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  175-85     Citation Subset:  IM; X    
Affiliation:
Department of Microbiology, Molecular Genetics and Immunology, Marion Merrell Dow Laboratory of Viral Pathogenesis, University of Kansas Medical Center, Kansas City 66160-7240, USA.
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MeSH Terms
Descriptor/Qualifier:
Acquired Immunodeficiency Syndrome / immunology,  pathology,  virology*
Amino Acid Sequence
Animals
Basal Ganglia / virology
Base Sequence
Brain / virology
CD4-Positive T-Lymphocytes / immunology*
DNA Primers
Genes, gag
HIV / genetics*,  isolation & purification,  pathogenicity
HIV Envelope Protein gp120 / biosynthesis,  chemistry,  genetics*
Kidney / virology
Lymph Nodes / virology
Macaca mulatta
Macaca nemestrina
Molecular Sequence Data
Polymerase Chain Reaction
Reassortant Viruses / genetics*,  isolation & purification,  pathogenicity
Sequence Homology, Amino Acid
Simian immunodeficiency virus / genetics*,  isolation & purification,  pathogenicity
Spleen / virology
Thymus Gland / pathology,  virology
Grant Support
ID/Acronym/Agency:
AI-29382/AI/NIAID NIH HHS; NS-32203/NS/NINDS NIH HHS; RR-06753/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/HIV Envelope Protein gp120

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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