Document Detail

Initial characterization of a peripheral blood mononuclear cell chemoattractant derived from cultured arterial smooth muscle cells.
MedLine Citation:
PMID:  6391189     Owner:  NLM     Status:  MEDLINE    
A mononuclear cell chemoattractant of high specific activity produced by baboon (Papio cynocephalus) aortic medial smooth-muscle cells (SMCs) in culture has been partially characterized. Smooth-muscle cells, between the third and eighth passage, were grown to confluence in Medium 199 containing 10% fetal calf serum and then incubated for 24 hours in either serumless medium (Neuman and Tytell) or Medium 199 containing 0.2% bovine serum albumin. The 24-hour SMC-conditioned medium was fractionated on Sephadex G100-Superfine and potent chemoattractant activity (SMC-CF) eluted in the 10,000-12,000 dalton region. SMC-CF displayed chemotactic and chemokinetic activity for peripheral blood mononuclear cells but not for polymorphonuclear leukocytes. Production of SMC-CF by the cells was significantly inhibited in the presence of cycloheximide, and its activity was abolished after incubation with the bacterial protease subtilisin. Chromatofocusing experiments indicate that SMC-CF is a cationic protein with a pI of greater than 10.5. The role of SMC-CF may play as an inflammatory mediator in monocyte recruitment to the arterial intima in atherogenesis is discussed.
A J Valente; S R Fowler; E A Sprague; J L Kelley; C A Suenram; C J Schwartz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of pathology     Volume:  117     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-01-14     Completed Date:  1985-01-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  409-17     Citation Subset:  AIM; IM    
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MeSH Terms
Chemotactic Factors / biosynthesis,  isolation & purification*,  physiology
Chemotaxis, Leukocyte / drug effects
Cycloheximide / pharmacology
Molecular Weight
Monocytes / metabolism*,  physiology
Muscle, Smooth, Vascular / cytology*,  metabolism
Neutrophils / physiology
Peptide Hydrolases / pharmacology
Grant Support
Reg. No./Substance:
0/Chemotactic Factors; 0/Interleukin-8; 66-81-9/Cycloheximide; EC 3.4.-/Peptide Hydrolases

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