| Initial characterization of a peripheral blood mononuclear cell chemoattractant derived from cultured arterial smooth muscle cells. | |
MedLine Citation:
|
PMID: 6391189 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A mononuclear cell chemoattractant of high specific activity produced by baboon (Papio cynocephalus) aortic medial smooth-muscle cells (SMCs) in culture has been partially characterized. Smooth-muscle cells, between the third and eighth passage, were grown to confluence in Medium 199 containing 10% fetal calf serum and then incubated for 24 hours in either serumless medium (Neuman and Tytell) or Medium 199 containing 0.2% bovine serum albumin. The 24-hour SMC-conditioned medium was fractionated on Sephadex G100-Superfine and potent chemoattractant activity (SMC-CF) eluted in the 10,000-12,000 dalton region. SMC-CF displayed chemotactic and chemokinetic activity for peripheral blood mononuclear cells but not for polymorphonuclear leukocytes. Production of SMC-CF by the cells was significantly inhibited in the presence of cycloheximide, and its activity was abolished after incubation with the bacterial protease subtilisin. Chromatofocusing experiments indicate that SMC-CF is a cationic protein with a pI of greater than 10.5. The role of SMC-CF may play as an inflammatory mediator in monocyte recruitment to the arterial intima in atherogenesis is discussed. |
Authors:
|
A J Valente; S R Fowler; E A Sprague; J L Kelley; C A Suenram; C J Schwartz |
Related Documents
:
|
23573019 - Enhanced shoot multiplication in ficus religiosa l. in the presence of adenine sulphate... 7378429 - Effects of metabolic inhibitors on the synthesis and release of lipoprotein lipase in c... 1804729 - In vitro gallbladder motility in patients with radiolucent and radiopaque stones. 3100319 - Apolipoprotein e synthesis in neurofibrosarcoma and schwannoma cell cultures from two i... 7037409 - Attachment of lipoprotein to the murein of escherichia coli. 2875319 - Atherogenicity of blood serum from patients with coronary heart disease. 3017789 - Growth hormone receptors in cultured adipocytes: a model to study receptor regulation. 21179429 - Physiological expression of lens α-, β-, and γ-crystallins in murine and human corneas. 1715499 - A simple method for organotypic cultures of nervous tissue. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The American journal of pathology Volume: 117 ISSN: 0002-9440 ISO Abbreviation: Am. J. Pathol. Publication Date: 1984 Dec |
Date Detail:
|
Created Date: 1985-01-14 Completed Date: 1985-01-14 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 409-17 Citation Subset: AIM; IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chemotactic Factors / biosynthesis, isolation & purification*, physiology Chemotaxis, Leukocyte / drug effects Cycloheximide / pharmacology Interleukin-8 Molecular Weight Monocytes / metabolism*, physiology Muscle, Smooth, Vascular / cytology*, metabolism Neutrophils / physiology Papio Peptide Hydrolases / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
1-P01-HL-26890/HL/NHLBI NIH HHS; HL-07446/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Chemotactic Factors; 0/Interleukin-8; 66-81-9/Cycloheximide; EC 3.4.-/Peptide Hydrolases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In vitro activation of murine Kupffer cells by lymphokines or endotoxins to lyse syngeneic tumor cel...
Next Document: Monocyte/macrophage-specific monoclonal antibody Ki-M1 recognizes interdigitating reticulum cells.