| Initial characterization of a peripheral blood mononuclear cell chemoattractant derived from cultured arterial smooth muscle cells. | |
MedLine Citation:
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PMID: 6391189 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A mononuclear cell chemoattractant of high specific activity produced by baboon (Papio cynocephalus) aortic medial smooth-muscle cells (SMCs) in culture has been partially characterized. Smooth-muscle cells, between the third and eighth passage, were grown to confluence in Medium 199 containing 10% fetal calf serum and then incubated for 24 hours in either serumless medium (Neuman and Tytell) or Medium 199 containing 0.2% bovine serum albumin. The 24-hour SMC-conditioned medium was fractionated on Sephadex G100-Superfine and potent chemoattractant activity (SMC-CF) eluted in the 10,000-12,000 dalton region. SMC-CF displayed chemotactic and chemokinetic activity for peripheral blood mononuclear cells but not for polymorphonuclear leukocytes. Production of SMC-CF by the cells was significantly inhibited in the presence of cycloheximide, and its activity was abolished after incubation with the bacterial protease subtilisin. Chromatofocusing experiments indicate that SMC-CF is a cationic protein with a pI of greater than 10.5. The role of SMC-CF may play as an inflammatory mediator in monocyte recruitment to the arterial intima in atherogenesis is discussed. |
Authors:
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A J Valente; S R Fowler; E A Sprague; J L Kelley; C A Suenram; C J Schwartz |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of pathology Volume: 117 ISSN: 0002-9440 ISO Abbreviation: Am. J. Pathol. Publication Date: 1984 Dec |
Date Detail:
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Created Date: 1985-01-14 Completed Date: 1985-01-14 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 409-17 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chemotactic Factors / biosynthesis, isolation & purification*, physiology Chemotaxis, Leukocyte / drug effects Cycloheximide / pharmacology Interleukin-8 Molecular Weight Monocytes / metabolism*, physiology Muscle, Smooth, Vascular / cytology*, metabolism Neutrophils / physiology Papio Peptide Hydrolases / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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1-P01-HL-26890/HL/NHLBI NIH HHS; HL-07446/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemotactic Factors; 0/Interleukin-8; 66-81-9/Cycloheximide; EC 3.4.-/Peptide Hydrolases |
| Comments/Corrections | |
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