Document Detail

Initial characterization of the microenvironment that regulates connective tissue degradation in amniochorion during normal human labor.
MedLine Citation:
PMID:  15955681     Owner:  NLM     Status:  MEDLINE    
Extracellular matrix degradation in fetal membranes leading to its rupture is coupled to myometrial activity and cervical ripening during human normal labor. Mechanisms which modulate collagen degradation in amniochorion during labor have not been elucidated. Initial characterization of the effect of different blood compartments on connective tissue degradation in amniochorion during human labor was explored. Amniochorion explants were stimulated with plasma of maternal venous blood, umbilical cord blood or placental blood, obtained from women with pregnancies at term, with or without labor. MMP-2 and MMP-9 activities were quantified in conditioned media by gelatin-zymography as an index of connective tissue degradation. Collagen content was measured in tissue explants and collagen fibrils distribution was examined by electron microscopy. Placental plasma from term pregnancies, with or without labor, is enriched with soluble signals that enhance the in vitro MMP-9 production by amniochorion. Accompanying ultrastructural distortion of collagen fibers and demonstration of collagen degradation fragments confirmed induction of extracellular matrix degradation. Control experiments in which MMP-9 activity was blocked with TIMP-1 resulted in inhibition of all the above mentioned changes. These results suggest that placental intervillous space is a functional compartment in which mediators capable to induce collagen degradation in amniochorion are selectively expressed during human labor.
Guadalupe Estrada-Gutierrez; Veronica Zaga; Marco Antonio Gonzalez-Jimenez; Jorge Beltran-Montoya; Rolando Maida-Claros; Silvia Giono-Cerezo; Felipe Vadillo-Ortega
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Matrix biology : journal of the International Society for Matrix Biology     Volume:  24     ISSN:  0945-053X     ISO Abbreviation:  Matrix Biol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-27     Completed Date:  2005-09-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9432592     Medline TA:  Matrix Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  306-12     Citation Subset:  IM    
Research Direction, Instituto Nacional de Perinatologia, Mexico City, Mexico.
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MeSH Terms
Amnion / cytology,  enzymology,  metabolism*
Chorion / cytology,  enzymology,  metabolism*
Connective Tissue / enzymology,  metabolism*
Labor, Obstetric / metabolism*
Leukocyte Count
Matrix Metalloproteinase 2 / genetics,  metabolism
Matrix Metalloproteinase 9 / genetics,  metabolism
Tissue Culture Techniques
Reg. No./Substance:
EC Metalloproteinase 2; EC Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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