Document Detail


Inhibitory and stimulatory signaling via immunoglobulin receptors: dichotomous responses elicited in clonal B cell populations.
MedLine Citation:
PMID:  1577065     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The paradox that cross-linking of IgM antigen receptors on B cells by native or surrogate antigen can inhibit, as well as stimulate, B cell functions has previously been attributed to changes during maturation and activation, programming either a negative or a positive response at defined developmental stages. In contrast to this concept, we show here that some B cells possess the potential for both types of response at the same stage. In three clonal malignant human B cell populations, bivalent soluble monoclonal antibodies to IgM or idiotype, but not IgD completely inhibited spontaneous DNA synthesis, but significantly induced [3H]thymidine uptake when coupled to insoluble compounds. In co-incubation experiments mitogenic stimuli were dominant over inhibitory ones and were still effective after prolonged pretreatment of the B cells with inhibitory reagents. Ionomycin, known to increase intracellular calcium levels, and low doses of phorbol ester, described to activate protein kinase C, also suppressed DNA synthesis. High doses of phorbol ester alone or in combination with ionomycin, however, induced DNA synthesis in two of the lymphomas. We conclude that some B cells may respond to cross-linking of surface IgM in a dose-dependent manner so that all signals increase DNA synthesis once a threshold has been reached. These dose-dependent effects may in part involve signaling via breakdown of membrane inositol phosphates.
Authors:
P M Van Endert; G Moldenhauer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunology     Volume:  22     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-06-05     Completed Date:  1992-06-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  1229-35     Citation Subset:  IM    
Affiliation:
Institute of Immunology and Genetics, German Cancer Research Center, Heidelberg.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology
B-Lymphocytes / immunology*
Calcium / physiology
Clone Cells
DNA / biosynthesis
Humans
Immunoglobulin D / physiology
Immunoglobulin Idiotypes / physiology
Immunoglobulin M / physiology
Lymphocyte Activation*
Protein Kinase C / physiology
Receptors, Antigen, B-Cell / physiology
Receptors, Fc / physiology*
Receptors, Immunologic / physiology*
Signal Transduction
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Immunoglobulin D; 0/Immunoglobulin Idiotypes; 0/Immunoglobulin M; 0/Receptors, Antigen, B-Cell; 0/Receptors, Fc; 0/Receptors, Immunologic; 0/immunoglobulin D receptor; 0/immunoglobulin M receptor; 7440-70-2/Calcium; 9007-49-2/DNA; EC 2.7.11.13/Protein Kinase C

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