Document Detail


Inhibitory role of RhoA on senescence-like growth arrest by a mechanism involving modulation of phosphatase activity.
MedLine Citation:
PMID:  17658517     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently, negative effects of phosphatase in tumorigenesis and metastasis have been suggested in various tumor types. In this study, we showed that RhoA activation modulated phosphatase during senescence-like arrest in human prostate cancer cells. Under senescence-inducing condition, decreased Erk phosphorylation was detected in caRhoA-transfected cells and inactivation of Erk, but not p38, prevented doxorubicin-induced cell senescence. Cells were induced to senescence by inhibition of phosphatase activity (VHR, MKP3, or PP2A) without additional cellular stress. Of interest, caRhoA prevented doxorubicin-induced decrease of phosphatase. Thus, we postulate that RhoA signaling may protect cells against cellular senescence by maintaining phosphatase activity and Erk dephosphorylation.
Authors:
Chaehwa Park; Inkyoung Lee; Jun Ho Jang; Won Ki Kang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-16
Journal Detail:
Title:  FEBS letters     Volume:  581     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-01     Completed Date:  2007-09-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3800-4     Citation Subset:  IM    
Affiliation:
Cancer Center, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-dong, Kangnam-gu, Seoul 135-710, Republic of Korea. cpark@smc.samsung.co.kr
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology
Cell Aging / physiology*
Cell Cycle / physiology
Cell Line, Tumor
Doxorubicin / pharmacology
Enzyme Activation
Humans
Male
Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors
Phosphoric Monoester Hydrolases / metabolism*
Phosphorylation
Prostatic Neoplasms / metabolism,  pathology
Transfection
rhoA GTP-Binding Protein / metabolism*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 23214-92-8/Doxorubicin; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.6.5.2/rhoA GTP-Binding Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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