| Inhibitory effects of various beverages on the sulfoconjugation of 17beta-estradiol in human colon carcinoma Caco-2 cells. | |
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MedLine Citation:
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PMID: 18981586 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To investigate the possible effects of different beverages in the gastrointestinal tract on the sulfation of estrogen, which is a major hormone and prototype substrate for the human sulfotransferases (SULT), we analyzed the effects of these substances upon the sulfate conjugation of 17beta-estradiol (E2) in the human colon carcinoma cell line Caco-2. Sulfoconjugation activity toward E2 was measured by incubating 20 nM E2 with Caco-2 cells in the presence (5% (v/v)) of each beverage. Among the 35 beverages analyzed, four (aronia, blueberry, coffee, and peppermint) exhibited strong inhibitory effects on E2 sulfation within Caco-2 cells IC50 values ranging from 1.9 to 4.4% (v/v)). These active beverages also strongly inhibited the cytosolic estrogen SULT activity of Caco-2 cells in vitro (IC50 values ranging from 0.18 to 0.3% (v/v)). These inhibitory activities were extractable with ethyl acetate but not hexane or n-butanol, indicating that the molecules responsible are moderately lipophilic. Coffee was found to be the most potent inhibitor but the major constituents of this beverage, caffeic acid, caffeine, and chlorogenic acid, did not show any effects on estrogen SULT activity. Kinetic analyses further indicated that the mode of inhibition by coffee is competitive. A possible relationship between the inhibition of estrogen SULT activity by coffee in the gastrointestinal tract and the reported reduction of colon cancer incidence in women who consume coffee is discussed. |
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Authors:
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Ayako Saruwatari; Marina Isshiki; Hiroomi Tamura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 31 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-04 Completed Date: 2009-01-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
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Languages: eng Pagination: 2131-6 Citation Subset: IM |
Affiliation:
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Graduate School of Pharmaceutical Sciences, Keio University, Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Beverages*
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analysis Caco-2 Cells Chromatography, Thin Layer Coffee Colonic Neoplasms / enzymology, metabolism, prevention & control Cytosol / enzymology, metabolism Estradiol / analogs & derivatives*, metabolism Gastrointestinal Tract* / enzymology, metabolism Humans Solvents / chemistry Sulfotransferases / metabolism* Tea |
| Chemical | |
Reg. No./Substance:
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0/Coffee; 0/Solvents; 0/Tea; 28814-94-0/estradiol sulfate; 50-28-2/Estradiol; EC 2.8.2.-/Sulfotransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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