Document Detail


Inhibitory effects of progestogens on the estrogen stimulation of melanocytes in vitro.
MedLine Citation:
PMID:  19698824     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The use of oral estrogen-progestogen contraceptives may cause melasma, an epidermal hyperpigmentation in sun-exposed areas of the face. It is assumed that elevated estrogen levels lead to the activation of melanocytes, while the role of the gestagen component of contraceptives in pigmentation remains unclear and may vary between the different progestogens. In this study, we analyzed the distinct effects of progesterone and chlormadinone acetate (CMA) on melanocytes in comparison with estrogen. STUDY DESIGN: Human melanocytes were exposed to different concentrations of 17beta-estradiol and progestogens and analyzed for proliferation by a fluorometric cell viability assay and for pigmentation by a (3)H-labeled tyrosine assay. Subgroups of cells were additionally irradiated with UVA or UVB. RESULTS: Proliferation of melanocytes was induced by 17beta-estradiol (0.1 and 1 nM) in approximately half of the experiments, while progesterone (100 nM) and CMA (100 nM) reduced the proliferation rate by 38% and 27%, respectively. The pigmentation activity was slightly stimulated by 17beta-estradiol, whereas progestogens had no effect on the tyrosinase activity. CONCLUSIONS: Our data suggest that progesterone and CMA can inhibit proliferation of human melanocytes, which counteracts the stimulatory effects of estrogen. This may be of relevance for the choice of progestogen in oral contraceptives to prevent the development of melasma.
Authors:
Christine Wiedemann; Ursula Nägele; Georg Schramm; Carola Berking
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-23
Journal Detail:
Title:  Contraception     Volume:  80     ISSN:  1879-0518     ISO Abbreviation:  Contraception     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-24     Completed Date:  2009-12-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0234361     Medline TA:  Contraception     Country:  United States    
Other Details:
Languages:  eng     Pagination:  292-8     Citation Subset:  IM    
Affiliation:
Department of Dermatology and Allergology, Ludwig-Maximilian University, Munich, Germany.
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MeSH Terms
Descriptor/Qualifier:
Cell Proliferation / drug effects,  radiation effects
Chlormadinone Acetate / pharmacology*
Contraceptives, Oral, Synthetic / pharmacology*
Drug Interactions
Estradiol / pharmacology*
Humans
Melanocytes / drug effects*,  metabolism,  radiation effects
Melanosis / etiology,  metabolism,  prevention & control
Monophenol Monooxygenase / metabolism
Pigmentation / drug effects,  physiology,  radiation effects
Progesterone / pharmacology*
Ultraviolet Rays
Chemical
Reg. No./Substance:
0/Contraceptives, Oral, Synthetic; 302-22-7/Chlormadinone Acetate; 50-28-2/Estradiol; 57-83-0/Progesterone; EC 1.14.18.1/Monophenol Monooxygenase

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