Document Detail

Inhibitory effects of phytopolyphenols on TPA-induced transformation, PKC activation, and c-jun expression in mouse fibroblast cells.
MedLine Citation:
PMID:  9290125     Owner:  NLM     Status:  MEDLINE    
The effects of 12 phytopolyphenols, including 3 catechin derivatives, 8 flavanols, and curcumin, on 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced transformation in mouse fibroblast cells are described. The location of hydroxyl functional groups at 2', 3', or 4' site(s), especially at the 4' site, as in apigenin, fisetin, morin, myricetin, and quercetin, seems essential for anti-TPA-induced transformation, anti-protein kinase C (PKC) activation, and anti-TPA-induced c-jun expression activities. Among the catechin derivatives, those with a cis form structure and with an additional hydroxyl group at the R1 site and galloyl groups at the R2 site, such as epigallocatechin gallate, have stronger inhibitory effects on the above-mentioned biochemical activities. Considering their polarities and chemical structures, these compounds have different oxygen radical absorbance capacity in the in vitro assay. In addition, curcumin also has these inhibitory effects on the above-mentioned biochemical activities and antioxidant activities for peroxyl radical but not hydroxyl radical. In addition to PKC activation, active oxygen species production, and c-jun induction, our data suggest that TPA treatment induces cellular transformation by other unknown routes, because some tested compounds have an inhibitory effect on TPA-induced transformation but have no or a slight inhibitory effect on PKC activation, active oxygen species production, and c-jun induction.
S F Lee; J K Lin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nutrition and cancer     Volume:  28     ISSN:  0163-5581     ISO Abbreviation:  Nutr Cancer     Publication Date:  1997  
Date Detail:
Created Date:  1997-12-17     Completed Date:  1997-12-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7905040     Medline TA:  Nutr Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  177-83     Citation Subset:  IM    
Institute of Biochemistry, College of Medicine, National Taiwan University, Taipei, Republic of China.
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MeSH Terms
3T3 Cells / drug effects,  enzymology,  pathology
Carcinogens / toxicity
Catechin / chemistry,  pharmacology
Cell Line
Cell Transformation, Neoplastic / chemically induced,  drug effects*,  genetics,  metabolism
Curcumin / chemistry,  pharmacology
Down-Regulation / drug effects
Fibroblasts / drug effects,  enzymology,  pathology
Flavonoids / chemistry,  pharmacology
Gene Expression Regulation, Neoplastic / drug effects
Genes, jun / drug effects,  genetics*
Phenols / chemistry,  pharmacology*
Polymers / chemistry,  pharmacology*
Protein Kinase C / drug effects,  metabolism*
Tetradecanoylphorbol Acetate / toxicity
Reg. No./Substance:
0/Carcinogens; 0/Flavonoids; 0/Flavonols; 0/Phenols; 0/Polymers; 154-23-4/Catechin; 16561-29-8/Tetradecanoylphorbol Acetate; 458-37-7/Curcumin; EC Kinase C

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