Document Detail


Inhibitory effects of a chemically standardized extract from Scutellaria barbata in human colon cancer cell lines, LoVo.
MedLine Citation:
PMID:  16218664     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Scutellaria barbata (SB) is a medicinal plant that contains flavonone compounds such as scutellarein, scutellarin, carthamidin, isocarthamidin, and wogonin. A functional proteomic approach was used to study the inhibitory effects of a chemically standardized extract from SB in human colon adrencarcinoma, LoVo. In this work, a stable isotope was not used in the proposed method developed. The whole cell lysates from the control and treated cells were digested with trypsin, and the peptides were separated by two-dimensional (cation-exchange and reversed-phase) liquid chromatography and tandem mass spectrometry. The differentially expressed proteins identified using the current approach supported the data obtained from cell-cycle analysis with flow cytometry. With flow-cytometry analysis, a significant increase in the sub G1 phase was observed with a higher dose of extract from SB. Our results suggest that the chemically standardized extract from SB can induce cell death in the human colon cancer cell line. Our current work showed that the proposed platform provided a rapid approach to study the molecular mechanism because of the inhibitory effects of different doses of the botanical extracts on LoVo cell lines. This included a network of proteins involved in metabolism, regulation of the cell cycle, and transcription-factor activity.
Authors:
David Goh; Yian Hoon Lee; Eng Shi Ong
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  53     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-12     Completed Date:  2005-12-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8197-204     Citation Subset:  IM    
Affiliation:
Applied Science School, Temasek Polytechnic, 21 Tampinese Avenue 1, Singapore 529757, Republic of Singapore.
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MeSH Terms
Descriptor/Qualifier:
Cell Death / drug effects
Cell Division / drug effects
Cell Line, Tumor
Chromatography, High Pressure Liquid
Colonic Neoplasms / pathology*
Flow Cytometry
G1 Phase / drug effects
Humans
Mass Spectrometry
Plant Extracts / administration & dosage,  pharmacology*
Proteomics
Scutellaria / chemistry*
Chemical
Reg. No./Substance:
0/Plant Extracts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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