Document Detail


Inhibitory effects of bulbocapnine on dopamine biosynthesis in PC12 cells.
MedLine Citation:
PMID:  9593514     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of bulbocapnine, an aporphine isoquinoline alkaloid, on dopamine biosynthesis in PC12 cells were investigated. Bulbocapnine showed 45.2% inhibition on dopamine content in PC12 cells at a concentration of 20 microM for 12 h. The IC50 value of bulbocapnine was 26.7 microM. Bulbocapnine at concentrations up to 80 microM was not cytotoxic towards PC12 cells. Tyrosine hydroxylase (TH) activity was inhibited by the treatment of bulbocapnine in PC12 cells (24.4% inhibition at 20 microM). Bulbocapnine at 20 microM also decreased the intracellular Ca2+ concentration by 12.9% inhibition relative to control in PC12 cells. However, TH mRNA level was not altered by bulbocapnine treatment. These results suggest that the inhibition of TH activity by bulbocapnine might be involved in at least one component of the reduction of dopamine biosynthesis in PC12 cells.
Authors:
J S Shin; K T Kim; M K Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  244     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-11-18     Completed Date:  1998-11-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  161-4     Citation Subset:  IM    
Affiliation:
College of Pharmacy, Chungbuk National University, Kaeshin-Dong, Cheongju, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aporphines / pharmacology*
Calcium / metabolism
Cyclic AMP / metabolism
Dopamine / biosynthesis*
Dopamine Antagonists / pharmacology
PC12 Cells
Psychotropic Drugs / pharmacology*
RNA, Messenger / metabolism
Rats
Tyrosine 3-Monooxygenase / genetics
Chemical
Reg. No./Substance:
0/Aporphines; 0/Dopamine Antagonists; 0/Psychotropic Drugs; 0/RNA, Messenger; 298-45-3/bulbocapnine; 60-92-4/Cyclic AMP; 7440-70-2/Calcium; EC 1.14.16.2/Tyrosine 3-Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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