Document Detail

Inhibitory effects of bark extracts from Ulmus laevis on endometrial carcinoma: an in-vitro study.
MedLine Citation:
PMID:  19337064     Owner:  NLM     Status:  MEDLINE    
Anti-inflammatory effects of elm tree have been shown in several studies. Besides this, protective effects of components of elm bark on damaged tissue have also been described. This study was carried out to investigate the antitumour potential of an ethanolic extract isolated from Ulmus laevis in the hormone-dependent endometrial carcinoma cell line RL95-2. A range of 2.5-500 microg/ml of elm bark extract was used as standard concentrations. The molecular-chemical composition of the bark extract was analysed by pyrolysis-field ionization mass spectrometry. The influence of the bark extracts was determined on cell vitality [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide test], cell proliferation (5-bromo-2-deoxyuridine test) and cytotoxicity (lactate dehydrogenase test) in the human endometrial carcinoma cell line RL 95-2. By pyrolysis-field ionization mass spectrometry, the main substance classes of the extract as a composition of sterols/triterpenes, free fatty acids and a group of phenols, lignin monomers and flavonoids was identified. Our study showed a significant inhibition of cell vitality and proliferation measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide test up to 5 microg/ml extract and up to 100 microg/ml according to the 5-bromo-2-deoxyuridine test. Concentrations of 500 microg/ml induced a significant inhibition of cell vitality up to 80% and cell proliferation up to 81.5%. A significant cytotoxity was not observed. The results lead to the assumption that the bark extract from Ulmus laevis has antiproliferation and anticancer potential in hormone-dependent endometrial carcinoma cells.
Daniel Paschke; Sibylle Abarzua; André Schlichting; Dagmar-Ulrike Richter; Peter Leinweber; Volker Briese
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)     Volume:  18     ISSN:  1473-5709     ISO Abbreviation:  Eur. J. Cancer Prev.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-01     Completed Date:  2009-07-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9300837     Medline TA:  Eur J Cancer Prev     Country:  England    
Other Details:
Languages:  eng     Pagination:  162-8     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Rostock, Südstadt Klinikum, Südring, Rostock, Western Pomerania, Germany.
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MeSH Terms
Carcinoma / drug therapy,  pathology*
Cell Death / drug effects
Cell Proliferation / drug effects*
Cell Survival / drug effects
Dose-Response Relationship, Drug
Down-Regulation / drug effects
Drug Evaluation, Preclinical
Endometrial Neoplasms / drug therapy,  pathology*
Estradiol / pharmacology
Plant Bark / chemistry
Plant Extracts / chemistry,  pharmacology*,  therapeutic use
Tamoxifen / pharmacology
Tumor Cells, Cultured
Ulmus* / chemistry
Reg. No./Substance:
0/Plant Extracts; 10540-29-1/Tamoxifen; 50-28-2/Estradiol

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