| Inhibitory effects of antisense phosphorothioate oligodeoxynucleotides on pancreatic cancer cell Bxpc-3 telomerase activity and cell growth in vitro. | |
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MedLine Citation:
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PMID: 16810748 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the effect of telomerase hTERT gene antisense oligonucleotide (hTERT-ASO) on proliferation and telomerase activity of pancreatic cancer cell line Bxpc-3. METHODS: MTT assay was used to detect the effect of different doses of hTERT-ASO on proliferation of Bxpc-3 cell for different times. To study the anti-tumor activity, the cells were divided into three groups: Control group (pancreatic cancer cell Bxpc-3); antisense oligonucleotide (hTERT-ASO) group; and nosense oligonucleotide group decorated with phosphorothioate. Telomerase activity was detected using TRAP-PCR-ELISA. Cell DNA distribution was examined using flow cytometry assay. Cell apoptosis was observed by transmission electron microscope in each group. RESULTS: After treatment with 6 mmol/L hTERT-ASO, cell proliferation was inhibited in dose- and time-dependent manner. The telomerase activity decreased after treatment with hTERT-ASO for 72 h. Flow cytometry showed the cell number of G0/G1 phase increased from 2.7% to 14.7%, the cell number of S phase decreased from 72.7% to 51.0%, and a sub-G1 stage cell apoptosis peak appeared in front of G1 stage. CONCLUSION: Telomerase antisense oligodeoxy-nucleotide can inhibit the proliferation of pancreatic cancer cell line Bxpc-3 and decrease the telomerase activity and increase cell apoptosis rate in vitro. |
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Authors:
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Yun-Feng Wang; Ke-Jian Guo; Bei-Ting Huang; Yong Liu; Xiao-Yun Tang; Jian-Jun Zhang; Qiang Xia |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 12 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-06-30 Completed Date: 2006-10-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 4004-8 Citation Subset: IM |
Affiliation:
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Department of General Surgery, The Center Hospital of Shanghai Yangpu District (Branch Hospital of the Affiliated Xinhua Hospital of Shanghai Jiaotong University), Shanghai 200127, China. wangyunfeng197911@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects Cell Line, Tumor Cell Proliferation / drug effects* DNA-Binding Proteins / genetics*, metabolism* Gene Therapy Humans Oligodeoxyribonucleotides, Antisense / pharmacology* Pancreatic Neoplasms / enzymology, pathology* Telomerase / genetics*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Oligodeoxyribonucleotides, Antisense; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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