Document Detail

Inhibitory effects of actinomycin D and cycloheximide on neuronal death in adult Manduca sexta.
MedLine Citation:
PMID:  8113783     Owner:  NLM     Status:  MEDLINE    
A decline in circulating 20-hydroxyecdysone permits the emergence of the adult Manduca sexta moth; this endocrine signal also triggers the death of approximately half of the neurons in the unfused abdominal ganglia of the moth central nervous system. This programmed death of neurons was markedly reduced by treatment with either actinomycin D (an RNA synthesis inhibitor) or cycloheximide (a protein synthesis inhibitor). Similar results were found after addition of these agents to ventral nerve cord cultures. The effectiveness of these treatments in delaying or blocking neuronal death depended upon their time of administration relative to the normal time of post-emergence death in the particular motoneuron under study: late-dying neurons, for example, could still be saved by these treatments even after early-dying neurons had already initiated degeneration. In both intact moths and cultured ventral nerve cords, the ability of actinomycin D to prevent neuronal death waned at the same time at which replacement of the steroid hormone could no longer block neuronal death. This suggests that the steroid commitment point represents the time at which the genes that mediate cell death are transcribed. Cycloheximide remained effective in delaying or blocking neuronal death until shortly before the onset of degeneration, suggesting that ongoing protein synthesis is essential for the initiation of the degeneration response.
S E Fahrbach; M K Choi; J W Truman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurobiology     Volume:  25     ISSN:  0022-3034     ISO Abbreviation:  J. Neurobiol.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-03-31     Completed Date:  1994-03-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0213640     Medline TA:  J Neurobiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  59-69     Citation Subset:  IM    
Department of Entomology, University of Illinois at Urbana-Champaign 61801.
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MeSH Terms
Cell Death / drug effects
Cells, Cultured
Cycloheximide / pharmacology*
Dactinomycin / pharmacology*
Ecdysterone / physiology
Ganglia, Invertebrate / cytology
Moths / physiology*
Motor Neurons / drug effects
Nerve Degeneration / drug effects
Nerve Tissue Proteins / biosynthesis
Neurons / drug effects*
Grant Support
Reg. No./Substance:
0/Nerve Tissue Proteins; 50-76-0/Dactinomycin; 5289-74-7/Ecdysterone; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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