| Inhibitory effects of Salvianolic acid B on apoptosis of Schwann cells and its mechanism induced by intermittent high glucose. | |
| | |
MedLine Citation:
|
PMID: 22036624 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
AIMS: To investigate protective effects of Salvianolic acid B (Sal B) on the intermittent high glucose (IHG)-induced oxidative stress, mitochondrial pathway activation and Schwann cell (SC) apoptosis in vitro. MAIN METHODS: SCs were primarily cultured and exposed to the different conditions. Apoptosis was confirmed by the Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method and concentration of 8-hydroxy-2-deoxy Guanosine (8-OHdG) was detected by Elisa. Intracellular ROS generation and mitochondrial transmembrane potential (ΔΨm) were detected by flow cytometry analysis. Quantitative real-time reverse transcriptase PCR was performed to analyze the expression levels of Bax and BcL-2. Western blot was performed to analyze the expression levels of some important transcription factors and proteins. KEY FINDINGS: Treatment with Sal B inhibited the IHG-induced oxidative stress by reducing ROS production and 8-OHdG levels, mitochondrial depolarization and apoptosis in SCs in a dose-dependent manner. Furthermore, treatment with Sal B down-regulated the IHG-induced release of cytochrome c, AIF nuclear translocation and Bax expression, but mitigated the IHG-mediated down-regulation of BcL-2 expression in SCs. In addition, treatment with Sal B attenuated the IHG-induced activation of caspase-9 and caspase-3 and minimized the cleavage of PARP in SCs. SIGNIFICANCE: Our results indicated that IHG induced SC apoptosis in both caspase-dependent and caspase-independent pathways by activating the mitochondrial pathway. Sal B inhibited the IHG-induced oxidative stress, activation of the mitochondrial pathway and apoptosis in SCs. |
| | |
Authors:
|
Lian-Qing Sun; Bing Xue; Xiao-Jin Li; Xuan Wang; Ling Qu; Ting-Ting Zhang; Jue Zhao; Bao-An Wang; Xiao-Man Zou; Yi-Ming Mu; Ju-Ming Lu |
Related Documents
:
|
20585604 - A hypothesis concerning a potential involvement of ceramide in apoptosis and acantholys... 16441504 - Dissecting ascaris glycosphingolipids for immunomodulatory moieties--the use of synthet... 17191114 - The role of membrane lipids in the induction of macrophage apoptosis by microparticles. 20382204 - Importance of cytochrome c redox state for ceramide-induced apoptosis of human mammary ... 21878544 - Macrophage phagocytosis: effects of environmental pollutants, alcohol, cigarette smoke,... 1649464 - Importance of cell-mediated immunity in marek's disease and other viral tumor diseases. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-10-20 |
Journal Detail:
|
Title: Life sciences Volume: - ISSN: 1879-0631 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-10-31 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0375521 Medline TA: Life Sci Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Inc. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Casted-immobilization downregulates glucocorticoid receptor expression in rat slow-twitch soleus mus...
Next Document: NCX1 and NCX3: Two new effectors of delayed preconditioning in brain ischemia.