Document Detail

Inhibitory effects of 12-O-tetradecanoylphorbol-13-acetate on dye leakage from single Novikoff cells and on dye transfer between reaggregated cell pairs.
MedLine Citation:
PMID:  9500119     Owner:  NLM     Status:  MEDLINE    
The inhibitory effects of 12-O-tetradeconylphorbol-13-acetate (TPA) on the Lucifer Yellow leakage in single (nondissociated and dissociated) Novikoff cells in the presence of ethyleneglycotetraacetic acid (EGTA) and transfer between reaggregated cell pairs were investigated. Under treatment with TPA, single Novikoff cells showed inhibition of the dye leakage in the presence of EGTA, especially after 60-min treatment. There were only slight differences between nondissociated and dissociated cells in these experiments. The dye transfer in reaggregated cell pairs were significantly inhibited after 30-min treatment with TPA. In pretreatment with TPA during the recovery period of reaggregation the dye transfer in cell pairs was completely blocked. It was suggested that TPA blocks the assembly of gap junction, while it had no remarkable effect on gating mechanism of the hemichannels.
T F Liu; A F Paulson; H Y Li; M M Atkinson; R G Johnson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods and findings in experimental and clinical pharmacology     Volume:  19     ISSN:  0379-0355     ISO Abbreviation:  Methods Find Exp Clin Pharmacol     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-04-27     Completed Date:  1998-04-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7909595     Medline TA:  Methods Find Exp Clin Pharmacol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  573-7     Citation Subset:  IM    
Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing, China.
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MeSH Terms
Cell Communication / drug effects
Cell Membrane Permeability / drug effects
Cell Separation
Chelating Agents
Egtazic Acid
Fluorescent Dyes / chemistry*,  metabolism
Gap Junctions / drug effects
Isoquinolines / chemistry*,  metabolism
Tetradecanoylphorbol Acetate / pharmacology*
Tumor Cells, Cultured / drug effects
Reg. No./Substance:
0/Chelating Agents; 0/Fluorescent Dyes; 0/Isoquinolines; 16561-29-8/Tetradecanoylphorbol Acetate; 67-42-5/Egtazic Acid; 77944-88-8/lucifer yellow

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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