| Inhibitory effect of vasoactive intestinal polypeptide (VIP) on experimental liver metastasis by murine colon 26-L5 carcinoma cells. | |
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MedLine Citation:
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PMID: 10063970 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We previously reported that vasoactive intestinal polypeptide (VIP) significantly inhibited Matrigel invasion and haptotactic migration of murine colon 26-L5 carcinoma in vitro. To extend our study, we investigated the inhibitory mechanisms of VIP on Matrigel invasion of colon 26-L5 carcinoma, and the effect on metastatic properties of the tumor cells. VIP inhibited the invasion of the tumor cells in a concentration-dependent manner without affecting their growth, and achieved approximately 50% reduction at 10(-6) M. VIP also suppressed cell motility with a similar inhibition rate to the invasion assay. Time course study revealed that the motility was reduced by 40% when the tumor cells were preincubated with 10(-6) M VIP for 3 h. In contrast, 6-h pretreatment with 10(-6) M VIP caused the increased ability of the adhesion to both fibronectin and laminin with a 50% enhancement. A large amount of VIP1 receptor transcripts was expressed in the cells, whereas VIP2 receptor was undetectable, by RT-PCR and subsequent Southern blot hybridization. A specific antagonist for VIP1 receptor reversed the suppressed motility induced by VIP. Cryostat sections showed that the 3-h pretreatment of tumor cells with VIP caused the reduction of the arrest in the livers at 6 h after the tumor inoculation into a portal vein of mice. VIP could prevent the experimental liver metastasis of the tumor cells in a dose-dependent manner. The cells pretreated with 10(-6) M VIP for 3 h also showed the reduced ability of the liver metastasis. These results suggest that VIP could block the invasion and the metastasis of colon 26-L5 carcinoma through suppression of their motility. |
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Authors:
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M Ogasawara; J Murata; K Ayukawa; I Saiki |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncology research Volume: 10 ISSN: 0965-0407 ISO Abbreviation: Oncol. Res. Publication Date: 1998 |
Date Detail:
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Created Date: 1999-05-10 Completed Date: 1999-05-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9208097 Medline TA: Oncol Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 361-70 Citation Subset: IM |
Affiliation:
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Department of Pathogenic Biochemistry, Research Institute for Wakan-yaku, Toyama Medical and Pharmaceutical University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Southern Cell Adhesion / drug effects Cell Movement / drug effects Colonic Neoplasms / pathology* Dose-Response Relationship, Drug Fibronectins / pharmacology Laminin / pharmacology Liver Neoplasms / prevention & control*, secondary* Mice Mice, Inbred BALB C Reverse Transcriptase Polymerase Chain Reaction Time Factors Tumor Cells, Cultured Vasoactive Intestinal Peptide / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Fibronectins; 0/Laminin; 37221-79-7/Vasoactive Intestinal Peptide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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