| Inhibitory effect of thiopental on ultra-rapid delayed rectifier K+ current in H9c2 cells. | |
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MedLine Citation:
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PMID: 16217144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using the whole-cell voltage clamp technique, we investigated the effects of thiopental on membrane currents in H9c2 cells, a cell line derived from embryonic rat heart. Thiopental blocked a rapidly activating, very slowly-inactivating ultra-rapid type I(Kur)-like outward K(+) current in a concentration-dependent manner. The half-maximal concentration (IC(50)) of thiopental was 97 microM with a Hill coefficient of 1.2. The thiopental-sensitive current was also blocked by high concentrations of nifedipine (IC(50) = 9.1 microM) and 100 microM chromanol 293B, a blocker of slowly activating delayed rectifier K+ current (I(Ks)), but was insensitive to E-4031, an inhibitor of rapidly activating delayed rectifier K(+) current (I(Kr)). TEA (tetraethylammonium) at 5 mM and 4-AP (4-aminopiridine) at 1 mM reduced the K(+) current to 30.8 +/- 12.2% and 20.5 +/- 6.5% of the control, respectively. Using RT-PCR, we detected mRNAs of Kv2.1, Kv3.4, Kv4.1, and Kv4.3 in H9c2 cells. Among those, Kv2.1 and Kv3.4 have I(Kur)-type kinetics and are therefore candidates for thiopental-sensitive K(+) channels in H9c2 cells. This is the first report showing that thiopental inhibits I(Kur). This effect of thiopental may be involved in its reported prolongation of cardiac action potentials. |
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Authors:
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Hideki Suzuki; Nobuo Momoi; Tomoyuki Ono; Sachiko Maeda; Yayoi Shikama; Isao Matsuoka; Hitoshi Suzuki; Junko Kimura |
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Publication Detail:
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Type: Journal Article Date: 2005-10-08 |
Journal Detail:
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Title: Journal of pharmacological sciences Volume: 99 ISSN: 1347-8613 ISO Abbreviation: J. Pharmacol. Sci. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-10-18 Completed Date: 2006-05-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101167001 Medline TA: J Pharmacol Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 177-84 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Fukushima Medical University, School of Medicine, Fukushima 960-1295, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anesthetics
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pharmacology* Animals Cell Line Chromans / pharmacology Delayed Rectifier Potassium Channels / antagonists & inhibitors*, genetics, metabolism Kinetics Membrane Potentials / drug effects Myocytes, Cardiac Nifedipine / pharmacology Potassium / metabolism* Potassium Channel Blockers / pharmacology RNA, Messenger / metabolism Rats Shab Potassium Channels / drug effects*, genetics, metabolism Shaw Potassium Channels / drug effects*, genetics, metabolism Sulfonamides / pharmacology Thiopental / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Anesthetics; 0/Chromans; 0/Delayed Rectifier Potassium Channels; 0/Kv3.4 protein, rat; 0/Potassium Channel Blockers; 0/RNA, Messenger; 0/Shab Potassium Channels; 0/Shaw Potassium Channels; 0/Sulfonamides; 163163-23-3/6-cyano-4-(N-ethylsulfonyl-N-methylamino)-3-hydroxy-2,2-dimethylchromane; 21829-25-4/Nifedipine; 7440-09-7/Potassium; 76-75-5/Thiopental |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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