Document Detail


Inhibitory effect of piperlonguminine on melanin production in melanoma B16 cell line by downregulation of tyrosinase expression.
MedLine Citation:
PMID:  16420250     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tyrosinase is a key enzyme for melanin biosynthesis, and hyperpigmentation disorders are associated with abnormal accumulation of melanin pigments, which can be improved by treatment with depigmenting agents. In the present study, piperlonguminine from Piper longum was discovered to inhibit melanin production in melanoma B16 cells stimulated with alpha-melanocyte stimulating hormone (alpha-MSH), 3-isobutyl-1-methylxanthine or protoporphyrin IX, where the compound exhibited stronger depigmenting efficacy than kojic acid. However, piperlonguminine did not affect 1-oleoyl-2-acetyl-sn-glycerol-induced melanogenesis and did not affect protein kinase C-mediated melanin production. Surprisingly, piperlonguminine did not inhibit the catalytic activity of cell-free tyrosinase from melanoma B16 cells but rather suppressed tyrosinase mRNA expression. This effect was attributed to the inhibitory action of piperlonguminine on alpha-MSH-induced signaling through cAMP to the cAMP responsive element binding protein that in turn regulates the expression of the microphthalmia-associated transcription factor, a key activator of the tyrosinase promoter. This study demonstrates that piperlonguminine is an efficient depigmenting agent with a novel mechanism of action.
Authors:
Kyeong-Soo Kim; Jeong Ah Kim; Seung-Yong Eom; Seung Ho Lee; Kyung Rak Min; Youngsoo Kim
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society     Volume:  19     ISSN:  0893-5785     ISO Abbreviation:  Pigment Cell Res.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-19     Completed Date:  2006-04-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8800247     Medline TA:  Pigment Cell Res     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  90-8     Citation Subset:  IM    
Affiliation:
College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University, Cheongju, Korea.
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MeSH Terms
Descriptor/Qualifier:
1-Methyl-3-isobutylxanthine / metabolism
Animals
Cell Line, Tumor
Cell Proliferation / drug effects
Cyclic AMP Response Element-Binding Protein / metabolism
Diglycerides / metabolism
Dioxolanes / chemistry,  metabolism*,  pharmacology
Down-Regulation
Humans
Melanins / biosynthesis*
Melanoma
Microphthalmia-Associated Transcription Factor / metabolism
Molecular Structure
Monophenol Monooxygenase / genetics,  metabolism*
Phosphodiesterase Inhibitors / metabolism
Piper / chemistry*
Plant Extracts / chemistry,  metabolism*,  pharmacology
Protoporphyrins / metabolism
RNA, Messenger / metabolism
alpha-MSH / metabolism
Chemical
Reg. No./Substance:
0/Cyclic AMP Response Element-Binding Protein; 0/Diglycerides; 0/Dioxolanes; 0/Melanins; 0/Microphthalmia-Associated Transcription Factor; 0/Phosphodiesterase Inhibitors; 0/Plant Extracts; 0/Protoporphyrins; 0/RNA, Messenger; 0/piperlonguminine; 28822-58-4/1-Methyl-3-isobutylxanthine; 553-12-8/protoporphyrin IX; 581-05-5/alpha-MSH; 86390-77-4/1-oleoyl-2-acetylglycerol; EC 1.14.18.1/Monophenol Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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