| Inhibitory effect of piperlonguminine on melanin production in melanoma B16 cell line by downregulation of tyrosinase expression. | |
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MedLine Citation:
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PMID: 16420250 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tyrosinase is a key enzyme for melanin biosynthesis, and hyperpigmentation disorders are associated with abnormal accumulation of melanin pigments, which can be improved by treatment with depigmenting agents. In the present study, piperlonguminine from Piper longum was discovered to inhibit melanin production in melanoma B16 cells stimulated with alpha-melanocyte stimulating hormone (alpha-MSH), 3-isobutyl-1-methylxanthine or protoporphyrin IX, where the compound exhibited stronger depigmenting efficacy than kojic acid. However, piperlonguminine did not affect 1-oleoyl-2-acetyl-sn-glycerol-induced melanogenesis and did not affect protein kinase C-mediated melanin production. Surprisingly, piperlonguminine did not inhibit the catalytic activity of cell-free tyrosinase from melanoma B16 cells but rather suppressed tyrosinase mRNA expression. This effect was attributed to the inhibitory action of piperlonguminine on alpha-MSH-induced signaling through cAMP to the cAMP responsive element binding protein that in turn regulates the expression of the microphthalmia-associated transcription factor, a key activator of the tyrosinase promoter. This study demonstrates that piperlonguminine is an efficient depigmenting agent with a novel mechanism of action. |
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Authors:
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Kyeong-Soo Kim; Jeong Ah Kim; Seung-Yong Eom; Seung Ho Lee; Kyung Rak Min; Youngsoo Kim |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society Volume: 19 ISSN: 0893-5785 ISO Abbreviation: Pigment Cell Res. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-01-19 Completed Date: 2006-04-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8800247 Medline TA: Pigment Cell Res Country: Denmark |
Other Details:
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Languages: eng Pagination: 90-8 Citation Subset: IM |
Affiliation:
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College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University, Cheongju, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Methyl-3-isobutylxanthine
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metabolism Animals Cell Line, Tumor Cell Proliferation / drug effects Cyclic AMP Response Element-Binding Protein / metabolism Diglycerides / metabolism Dioxolanes / chemistry, metabolism*, pharmacology Down-Regulation Humans Melanins / biosynthesis* Melanoma Microphthalmia-Associated Transcription Factor / metabolism Molecular Structure Monophenol Monooxygenase / genetics, metabolism* Phosphodiesterase Inhibitors / metabolism Piper / chemistry* Plant Extracts / chemistry, metabolism*, pharmacology Protoporphyrins / metabolism RNA, Messenger / metabolism alpha-MSH / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cyclic AMP Response Element-Binding Protein; 0/Diglycerides; 0/Dioxolanes; 0/Melanins; 0/Microphthalmia-Associated Transcription Factor; 0/Phosphodiesterase Inhibitors; 0/Plant Extracts; 0/Protoporphyrins; 0/RNA, Messenger; 0/piperlonguminine; 28822-58-4/1-Methyl-3-isobutylxanthine; 553-12-8/protoporphyrin IX; 581-05-5/alpha-MSH; 86390-77-4/1-oleoyl-2-acetylglycerol; EC 1.14.18.1/Monophenol Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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