Document Detail

Inhibitory effect of phospholipase A(2) isolated from Crotalus durissus terrificus venom on macrophage function.
MedLine Citation:
PMID:  15777963     Owner:  NLM     Status:  MEDLINE    
Recent work demonstrated that crotoxin, the main toxin of Crotalus durissus terrificus venom, inhibits macrophage spreading and phagocytic activities. The crotoxin molecule is composed of two subunits, an acidic non-toxic and non-enzymatic polypeptide named crotapotin and a weakly toxic basic phospholipase A(2) (PLA(2)). In the present work, the active subunit responsible for the inhibitory effect of crotoxin on macrophage function was investigated. Peritoneal macrophages harvested from naive rats were used. Crotapotin (2.12, 3.75, or 8.37nM/ml), added for 2h to the medium of peritoneal cell incubation, did not modify the spreading and phagocytic activities of these cells. On the other hand, the PLA(2) (1.43, 2.86, or 6.43nM/ml) subunit caused a significant reduction (30, 33, and 35%, respectively) of the spreading activity. The PLA(2) also inhibited the phagocytosis of opsonised zymosan, opsonised sheep erythrocytes, and Candida albicans, indicating that this inhibitory effect is not dependent on the type of receptor involved in the phagocytosis process. The inhibitory effect of PLA(2) was not due to loss of cell membrane integrity, since macrophage viability was higher than 95%. These findings indicate that the inhibitory effect of crotoxin on macrophage spreading and phagocytic activities is caused by the phospholipase A(2) subunit.
S C Sampaio; A C Rangel-Santos; C M Peres; R Curi; Y Cury
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  45     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-21     Completed Date:  2005-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  671-6     Citation Subset:  IM    
Laboratory of Pathophysiology, Butantan Institute, Av. Vital Brazil, 1500, 05503-900 São Paulo, Brazil.
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MeSH Terms
Analysis of Variance
Candida albicans / metabolism
Crotalid Venoms / enzymology*
Dose-Response Relationship, Drug
Erythrocytes / metabolism
Macrophages / drug effects*
Phagocytosis / drug effects*
Phospholipases A / toxicity*
Rats, Wistar
Zymosan / metabolism
Reg. No./Substance:
0/Crotalid Venoms; 9010-72-4/Zymosan; EC 3.1.1.-/Phospholipases A

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