| Inhibitory effect of immature plum on PMA-induced MMP-9 expression in human hepatocellular carcinoma. | |
| | |
MedLine Citation:
|
PMID: 19418353 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The goal of this study was to evaluate the anticancer effect of Prunus salicina Lindl. cv. Soldam at three maturity stages (immature, midmature and mature). In search for anticancer effects of immature plum extract (IPE), we have found its antimigrative property in (phorbol 12-myristate 13-acetate) PMA-induced HepG2 cells, and this effect is associated with inhibition of MMP-9 activity. IPE appeared to have a strong inhibitory effect on the PMA-induced MMP-9 secretion through suppression of the transcriptional activity of the MMP-9 gene independently of the TIMP gene in HepG2 cells. PMA induced the translocation of c-jun and p65 to the nucleus; however, IPE inhibited their nuclear translocations induced by PMA in HepG2 cells. These results clearly indicate that IPE suppresses both AP-1- and NF-kappaB-mediated MMP-9 gene transcriptional activity through inhibiting the nuclear translocations of AP-1 and NF-kappaB. These findings suggest that AP-1 and NF-kappaB activations through the ERK, p38 MAPK and JNK pathways appears to be required for the induction of MMP-9 expression by PMA in IPE, and IPE regulates PMA-stimulated MMP-9 expression by suppressing the p38 MAPK, JNK and ERK pathways. IPE leads to a decrease in the migration potential of HepG2 cells in vitro, and this suggests that the migration inhibition is correlated well with its inhibition of MMP-9 expression. |
| | |
Authors:
|
Mi Hee Yu; Hyo Gwon Im; Sung Gyu Lee; Dae-Ik Kim; Hwa Jeong Seo; In-Seon Lee |
Related Documents
:
|
9211353 - In situ hybridization studies of matrix metalloproteinase-3, tissue inhibitor of metall... 12923463 - Prolonged spinal loading induces matrix metalloproteinase-2 activation in intervertebra... 21221513 - Rad is a p53 direct transcriptional target that inhibits cell migration and is frequent... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Natural product research Volume: 23 ISSN: 1478-6427 ISO Abbreviation: Nat. Prod. Res. Publication Date: 2009 |
Date Detail:
|
Created Date: 2009-05-06 Completed Date: 2009-07-23 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101167924 Medline TA: Nat Prod Res Country: England |
Other Details:
|
Languages: eng Pagination: 704-18 Citation Subset: IM |
Affiliation:
|
Department of Food Science and Technology and The Center for Traditional Microorganism Resources (TMR), Keimyung University, Daegu, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biological Transport
/
drug effects Blotting, Western Carcinoma, Hepatocellular / metabolism* Cell Line, Tumor Cell Movement / drug effects Gene Expression / drug effects* Humans Matrix Metalloproteinase 9 / genetics* NF-kappa B / metabolism Plant Extracts / pharmacology* Proto-Oncogene Proteins c-jun / metabolism Prunus / chemistry* Reverse Transcriptase Polymerase Chain Reaction Tetradecanoylphorbol Acetate / pharmacology* Transcription Factor AP-1 / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/NF-kappa B; 0/Plant Extracts; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factor AP-1; 16561-29-8/Tetradecanoylphorbol Acetate; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Biological activities of procyanidins from the bark of Pinus caribaea Morelet.
Next Document: Biological activity of alkaloids from Solanum dulcamara L.