Document Detail

Inhibitory effect of immature plum on PMA-induced MMP-9 expression in human hepatocellular carcinoma.
MedLine Citation:
PMID:  19418353     Owner:  NLM     Status:  MEDLINE    
The goal of this study was to evaluate the anticancer effect of Prunus salicina Lindl. cv. Soldam at three maturity stages (immature, midmature and mature). In search for anticancer effects of immature plum extract (IPE), we have found its antimigrative property in (phorbol 12-myristate 13-acetate) PMA-induced HepG2 cells, and this effect is associated with inhibition of MMP-9 activity. IPE appeared to have a strong inhibitory effect on the PMA-induced MMP-9 secretion through suppression of the transcriptional activity of the MMP-9 gene independently of the TIMP gene in HepG2 cells. PMA induced the translocation of c-jun and p65 to the nucleus; however, IPE inhibited their nuclear translocations induced by PMA in HepG2 cells. These results clearly indicate that IPE suppresses both AP-1- and NF-kappaB-mediated MMP-9 gene transcriptional activity through inhibiting the nuclear translocations of AP-1 and NF-kappaB. These findings suggest that AP-1 and NF-kappaB activations through the ERK, p38 MAPK and JNK pathways appears to be required for the induction of MMP-9 expression by PMA in IPE, and IPE regulates PMA-stimulated MMP-9 expression by suppressing the p38 MAPK, JNK and ERK pathways. IPE leads to a decrease in the migration potential of HepG2 cells in vitro, and this suggests that the migration inhibition is correlated well with its inhibition of MMP-9 expression.
Mi Hee Yu; Hyo Gwon Im; Sung Gyu Lee; Dae-Ik Kim; Hwa Jeong Seo; In-Seon Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Natural product research     Volume:  23     ISSN:  1478-6427     ISO Abbreviation:  Nat. Prod. Res.     Publication Date:  2009  
Date Detail:
Created Date:  2009-05-06     Completed Date:  2009-07-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101167924     Medline TA:  Nat Prod Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  704-18     Citation Subset:  IM    
Department of Food Science and Technology and The Center for Traditional Microorganism Resources (TMR), Keimyung University, Daegu, Republic of Korea.
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MeSH Terms
Biological Transport / drug effects
Blotting, Western
Carcinoma, Hepatocellular / metabolism*
Cell Line, Tumor
Cell Movement / drug effects
Gene Expression / drug effects*
Matrix Metalloproteinase 9 / genetics*
NF-kappa B / metabolism
Plant Extracts / pharmacology*
Proto-Oncogene Proteins c-jun / metabolism
Prunus / chemistry*
Reverse Transcriptase Polymerase Chain Reaction
Tetradecanoylphorbol Acetate / pharmacology*
Transcription Factor AP-1 / metabolism
Reg. No./Substance:
0/NF-kappa B; 0/Plant Extracts; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factor AP-1; 16561-29-8/Tetradecanoylphorbol Acetate; EC Metalloproteinase 9

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