Document Detail

Inhibitory effect of ibudilast (KC-404) on the expression of the beta2 integrin family on an eosinophilic cell line (EoL-1).
MedLine Citation:
PMID:  9860039     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Adhesion molecules are thought to play a key role in inflammatory processes in bronchial asthma. We previously observed an increased expression of the beta2-integrin family on an eosinophilic cell line (EoL-1) by platelet-activating factor (PAF). OBJECTIVE: In the present study, we examined the effect of ibudilast (KC-404), a novel anti-asthma agent, on beta2 integrin expression induced by PAF. MATERIAL AND METHODS: EoL-1 cells (1x10(6)/mL) were incubated in the presence or absence of 10(-6) M ibudilast (KC-404), then cells were cultured in the presence or absence of PAF (10(-7) M) for 45 minutes. Flow cytometric analysis for CD11a, CD11b, and CD18 expression was examined. RESULTS: Ibudilast had an inhibitory effect on beta2 integrin expression induced by PAF [CD11a: 84.8% versus 73.1% (preincubation with ibudilast), CD11b: 35.8% versus 26.2%, CD18 74.9% versus 65.6%]. CONCLUSIONS: Ibudilast (KC-404) has anti-inflammatory activities through its inhibitory effect on the expression of adhesion molecules on eosinophils.
J Chihara; O Urayama; H Kayaba; K Honda; N Saito; N Hayashi; D Kurachi; T Yamamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology     Volume:  81     ISSN:  1081-1206     ISO Abbreviation:  Ann. Allergy Asthma Immunol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1998-12-31     Completed Date:  1998-12-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9503580     Medline TA:  Ann Allergy Asthma Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  448-50     Citation Subset:  IM    
Department of Clinical and Laboratory Medicine, Akita University School of Medicine, Hondo, Japan.
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MeSH Terms
Antigens, CD18 / biosynthesis
Asthma / drug therapy
Bronchodilator Agents / pharmacology*
Integrins / biosynthesis*,  drug effects
Lymphocyte Function-Associated Antigen-1 / biosynthesis
Macrophage-1 Antigen / biosynthesis
Platelet Activating Factor / pharmacology
Platelet Aggregation Inhibitors / pharmacology
Pyridines / pharmacology*
Tumor Cells, Cultured / metabolism
Reg. No./Substance:
0/Antigens, CD18; 0/Bronchodilator Agents; 0/Integrins; 0/Lymphocyte Function-Associated Antigen-1; 0/Macrophage-1 Antigen; 0/Platelet Activating Factor; 0/Platelet Aggregation Inhibitors; 0/Pyridines; 50847-11-5/ibudilast

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