| Inhibitory effect of homochlorcyclizine on melanogenesis in α-melanocyte stimulating hormone-stimulated mouse B16 melanoma cells. | |
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MedLine Citation:
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PMID: 22297750 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The histamine receptor H1 antagonist homochlorcyclizine (HC) has been widely used as an antihistamine agent for the treatment of allergies. However, the effect of HC on skin pigmentation is not known. In the present study, we investigated the inhibitory effect of HC on melanogenesis in mouse B16 melanoma cells. Our results showed that HC inhibited melanogenesis in either α-melanocyte stimulating hormone (α-MSH)- or 3-isobutyl-1-methylxanthin (IBMX)-stimulated B16 cells in a dose-dependent manner. Despite the strong inhibition of melanogenesis by HC, it was surprisingly found that HC did not reduce either cellular or melanosomal tyrosinase activity in α-MSH-stimulated B16 cells. In addition, HC also did not directly inhibit either murine or mushroom tyrosinase activity in the cell-free system. Moreover, western blotting and reverse-transcription polymerase chain reaction (RT-PCR) analyses respectively confirmed that HC did not downregulate levels of tyrosinase protein and its mRNA in α-MSH-stimulated B16 cells. These results clearly demonstrated that HC inhibits melanogenesis of B16 cells by a mechanism other than reduction of the cellular tyrosinase activity. From the present study, HC was proven to be a good candidate as a skin-whitening agent for treatment of skin hyperpigmentation, and this generic drug might be suitable for use in combination with other depigmenting agents due to its unique inhibition mechanism. |
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Authors:
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Te-Sheng Chang; Chin-Tsun Chen |
Publication Detail:
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Type: Journal Article Date: 2012-02-02 |
Journal Detail:
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Title: Archives of pharmacal research Volume: 35 ISSN: 0253-6269 ISO Abbreviation: Arch. Pharm. Res. Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-02-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000036 Medline TA: Arch Pharm Res Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 119-27 Citation Subset: IM |
Affiliation:
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Department of Biological Science and Technology, National University of Tainan, Tainan, Taiwan, mozyme2001@gmail.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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