Document Detail


Inhibitory effect of ethidium bromide on mitotic chromosome condensation and its application to high-resolution chromosome banding.
MedLine Citation:
PMID:  6705566     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ethidium bromide (EB) is known to intercalate between stacked base pairs without specific base-pair preference. Its use in cultured human lymphocytes and Burkitt's lymphoma cells resulted in the accumulation of cells in prophase and prometaphase stages. Inhibition of mitotic chromosome condensation as a possible mechanism involved in this phenomenon is discussed. A simple method for obtaining high-resolution banding patterns on elongated chromosomes was devised as follows: Human lymphocytes cultured for 3 days with phytohemagglutinin were exposed to EB (5-10 micrograms/ml) and Colcemid (0.02 micrograms/ml) simultaneously for 2 h and then routinely harvested for chromosome preparation. High-resolution G-bands were obtained by Giemsa staining following mild trypsin treatment.
Authors:
T Ikeuchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  38     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1984  
Date Detail:
Created Date:  1984-05-23     Completed Date:  1984-05-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  56-61     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Burkitt Lymphoma / genetics
Cell Line
Cells, Cultured
Chromosome Banding
Ethidium / toxicity*
Humans
Karyotyping
Lymphocytes / drug effects,  physiology*
Mitosis / drug effects*
Trypsin
Chemical
Reg. No./Substance:
3546-21-2/Ethidium; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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