| Inhibitory effect of epigallocatechin-3-gallate on growth and invasion in human biliary tract carcinoma cells. | |
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MedLine Citation:
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PMID: 12053219 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Based on recent evidence that tea consumption contributes to a decreased incidence of human carcinomas, a number of investigators have focused on the mechanisms of cancer prevention by tea extracts, especially green tea polyphenols. Epigallocatechin-3-gallate (EGCG) is a representative polyphenol that inhibits the activity of the cyclin-dependent kinases of cdk2 and cdk4. This suggests that EGCG may exert its growth-inhibitory effects through modulation of G1 regulatory proteins such as cdk2 and cdk4. The human biliary tract carcinoma cells (TGBC-2, SK-ChA-1, and NOZC-1) were treated with different doses of EGCG (0, 25, 50, 100, and 200 mM) for 48 hours in cell medium. Cell proliferation was analyzed by WST-1 colorimetric assay. For the cell-invasion analysis, the cells were incubated with 100 mM of EGCG for 2 hours. The cells were then added into a Matrigel-coated Cell Insert. After incubation at 37 degrees C for 24 hours, the cells visible through the Matrigel were counted under the microscope. All human biliary tract cancer cells studied showed a significant suppression of cell growth by EGCG treatment in a dose-dependent manner (27.2%, 16.0%, and 10.1%, in TGBC-2, SK-ChA-1, and NOZC-1, respectively, at the dose of 200 mM). Epigallocatechin-3-gallate treatment also produced a significant suppression of invasive ability of the carcinoma cells (12.6%, 11.2%, 7.9%, in TGBC-2, SK-ChA-1, and NOZC-1, respectively, at a dose of 100 mM). These data indicated that EGCG might be a potent biological inhibitor of human biliary tract cancers, reducing their proliferative and invasive activities. |
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Authors:
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Moriatsu Takada; Yonson Ku; Kazuto Habara; Tetsuo Ajiki; Yasuyuki Suzuki; Yoshikazu Kuroda |
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Publication Detail:
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Type: Journal Article Date: 2002-03-01 |
Journal Detail:
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Title: World journal of surgery Volume: 26 ISSN: 0364-2313 ISO Abbreviation: World J Surg Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-06-07 Completed Date: 2002-08-30 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7704052 Medline TA: World J Surg Country: United States |
Other Details:
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Languages: eng Pagination: 683-6 Citation Subset: IM |
Affiliation:
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First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents, Phytogenic
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pharmacology* Bile Duct Neoplasms / drug therapy, pathology* Catechin / analogs & derivatives, pharmacology* Cell Division / drug effects* Drug Screening Assays, Antitumor Humans Neoplasm Invasiveness Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 154-23-4/Catechin; 989-51-5/epigallocatechin gallate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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