Document Detail

Inhibitory effect of epigallocatechin-3-gallate on growth and invasion in human biliary tract carcinoma cells.
MedLine Citation:
PMID:  12053219     Owner:  NLM     Status:  MEDLINE    
Based on recent evidence that tea consumption contributes to a decreased incidence of human carcinomas, a number of investigators have focused on the mechanisms of cancer prevention by tea extracts, especially green tea polyphenols. Epigallocatechin-3-gallate (EGCG) is a representative polyphenol that inhibits the activity of the cyclin-dependent kinases of cdk2 and cdk4. This suggests that EGCG may exert its growth-inhibitory effects through modulation of G1 regulatory proteins such as cdk2 and cdk4. The human biliary tract carcinoma cells (TGBC-2, SK-ChA-1, and NOZC-1) were treated with different doses of EGCG (0, 25, 50, 100, and 200 mM) for 48 hours in cell medium. Cell proliferation was analyzed by WST-1 colorimetric assay. For the cell-invasion analysis, the cells were incubated with 100 mM of EGCG for 2 hours. The cells were then added into a Matrigel-coated Cell Insert. After incubation at 37 degrees C for 24 hours, the cells visible through the Matrigel were counted under the microscope. All human biliary tract cancer cells studied showed a significant suppression of cell growth by EGCG treatment in a dose-dependent manner (27.2%, 16.0%, and 10.1%, in TGBC-2, SK-ChA-1, and NOZC-1, respectively, at the dose of 200 mM). Epigallocatechin-3-gallate treatment also produced a significant suppression of invasive ability of the carcinoma cells (12.6%, 11.2%, 7.9%, in TGBC-2, SK-ChA-1, and NOZC-1, respectively, at a dose of 100 mM). These data indicated that EGCG might be a potent biological inhibitor of human biliary tract cancers, reducing their proliferative and invasive activities.
Moriatsu Takada; Yonson Ku; Kazuto Habara; Tetsuo Ajiki; Yasuyuki Suzuki; Yoshikazu Kuroda
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Publication Detail:
Type:  Journal Article     Date:  2002-03-01
Journal Detail:
Title:  World journal of surgery     Volume:  26     ISSN:  0364-2313     ISO Abbreviation:  World J Surg     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-07     Completed Date:  2002-08-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7704052     Medline TA:  World J Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  683-6     Citation Subset:  IM    
First Department of Surgery, Kobe University School of Medicine, 7-5-2, Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
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MeSH Terms
Antineoplastic Agents, Phytogenic / pharmacology*
Bile Duct Neoplasms / drug therapy,  pathology*
Catechin / analogs & derivatives,  pharmacology*
Cell Division / drug effects*
Drug Screening Assays, Antitumor
Neoplasm Invasiveness
Tumor Cells, Cultured
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 154-23-4/Catechin; 989-51-5/epigallocatechin gallate

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