Document Detail

Inhibitory effect of eight simple coumarins on the lucigenin enhanced chemiluminescence of rabbit neutrophils.
MedLine Citation:
PMID:  14632318     Owner:  NLM     Status:  MEDLINE    
The generation of superoxide anion (O2*-) and other reactive oxygen species (ROS), such as HO*, HOCl, NO, 1O2 and H2O2, by stimulated polymorphonuclear leukocytes during phagocytosis is a major mechanism of host defense against invading microorganisms. However, large amounts of ROS are suggested to be responsible for many diseases. In this work we studied the inhibitory effect of eight simple coumarins on O2*- generation by rabbit neutrophil upon stimulation with opsonized zymosan, using lucigenin enhanced chemiluminescence assay. We observed that coumarins containing hydroxy or acetoxy substituents at position 7 of the benzopyrone ring were the most active ones (IC50 values ranging from 6.0 +/- 2.8 to 18.6 +/- 2.6 micromol/L). Substitution of these groups by allyloxy or metoxy groups decreased the activity and unsubstituted coumarin had no effect. Cell damage after exposure to 200 micromol/L of each compound was determined by measurement of the activity of the released cytosolic lactate dehydrogenase and by Trypan Blue dye exclusion test. None of the drugs affected significantly the cellular viability.
Luciana M Kabeya; Alexandre Kanashiro; Ana E C S Azzolini; Frederico M Soriani; João L C Lopes; Yara M Lucisano-Valim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Research communications in molecular pathology and pharmacology     Volume:  111     ISSN:  1078-0297     ISO Abbreviation:  Res. Commun. Mol. Pathol. Pharmacol.     Publication Date:  2002  
Date Detail:
Created Date:  2003-11-24     Completed Date:  2004-07-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9437512     Medline TA:  Res Commun Mol Pathol Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  103-13     Citation Subset:  IM    
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo. Av. do Café s/n, Ribeirão Preto, SP, Brasil, CEP 14040-903.
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MeSH Terms
Chemiluminescent Measurements
Coumarins / pharmacology*
Neutrophils / drug effects*,  metabolism
Superoxides / metabolism
Reg. No./Substance:
0/Acridines; 0/Coumarins; 11062-77-4/Superoxides; 2315-97-1/10,10'-dimethyl-9,9'-biacridinium

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